Hoechst 34580

• CAT Number: I002789
• CAS Number: 23555-00-2
• Molecular Formula: C27H29N7
• Molecular Weight: 451.57
• Purity: ≥95%
• Synonyms: N,N-dimethyl-4-(6-(4-methylpiperazin-1-yl)-1H,3/’H-[2,5/’-bibenzo[d]imidazol]-2/’-yl)aniline
• Target: Amyloid-β
• Solubility: DMSO or water Protect from light
• Storage: Store at -20°C

Hoechst 34580 (CAT: I002789) is a fluorescent dye that is widely used for DNA staining in biological research. It is a cell-permeable dye that selectively binds to DNA, allowing for the visualization and quantification of DNA in various applications such as fluorescence microscopy, flow cytometry, and gel electrophoresis. Hoechst 34580 emits blue fluorescence upon binding to DNA and can be used in combination with other fluorophores for multiplex staining. Its high affinity for DNA and low cytotoxicity make it a valuable tool for studying nuclear morphology, cell cycle analysis, and DNA content determination.

Reference

[1]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4/’,6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68.

[2]. a b c /Hoechst Stains/. Invitrogren (Molecular Probes).

[3]. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505.