Hygromycin B

• CAT Number: A000977
• CAS Number: 31282-04-9
• Molecular Formula: C20H37N3O13
• Molecular Weight: 527.50
• Purity: ≥95%
• Storage: 3 years -20C powder
• IUPAC Name: (2S,3'R,3aS,4S,4'S,5'R,6R,6'R,7S,7aS)-4-[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1R)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
• InChI: InChI=1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• InChIKey: GRRNUXAQVGOGFE-XKIAHZFYSA-N
• SMILES: CN[C@H]1C[C@H]([C@@H]([C@H]([C@@H]1O)O[C@H]2[C@@H]3[C@H]([C@H]([C@H](O2)CO)O)O[C@]4(O3)[C@@H]([C@H]([C@H]([C@H](O4)[C@@H](CO)N)O)O)O)O)N

Hygromycin B（Cat No.:A000977）is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus, widely used in molecular biology for selecting genetically modified cells. It inhibits protein synthesis by binding to the 30S ribosomal subunit, causing translation errors that lead to cell death. Hygromycin B is effective against a range of prokaryotic and eukaryotic organisms, making it suitable for use in creating transgenic plants, yeast, and mammalian cells. Its reliable selection efficiency allows researchers to identify successfully modified cells, making it a key tool in genetic engineering and research applications.

Reference

Dai S., Zheng P., Marmey P., Zhang S., Tian W., Chen S., Beachy R.N. and Fauquet C. Comparative analysis of transgenic rice plants obtained by Agrobacterium-mediated transformation and particle bombardment. Molecular Breeding 7: 25–33, 2001. © 2001 Kluwer Academic Publishers.Schindler, D. /Studies on the Mode of Action of Hygromycin B, an Inhibitor of Translocation in Eukaryotes./ Nucleic Acids and Protein Synthesis 521.2 (1978): 459-69. www.ncbi.gov. Web. 6 Sept. 2012.