IMM-01

• CAT Number: I012560
• CAS Number: 218795-74-5
• Molecular Formula: C12H17N3O2S
• Molecular Weight: 267.35
• Purity: ≥95%
• Synonyms: 1-tert-butyl-3-[(E)-(2,4-dihydroxyphenyl)methylideneamino]thiourea
• InChI: InChI=1S/C12H17N3O2S/c1-12(2,3)14-11(18)15-13-7-8-4-5-9(16)6-10(8)17/h4-7,16-17H,1-3H3,(H2,14,15,18)/b13-7+
• InChIKey: LTFUAYRGVLQXKC-NTUHNPAUSA-N
• SMILES: CC(C)(C)NC(=S)NN=CC1=C(C=C(C=C1)O)O

IMM-01 is a mammalian Diaphanous (mDia)-related formins agonist that inhibits DID-DAD (diaphanous inhibitory domain-diaphanous autoregulatory domain) binding with an IC50 of 140 nM. IMM-01 acts by disrupting the autoinhibitory bond between the DID and DAD domain and thus activates formins. IMM-01 shows anticancer effects[1].
IMM-01 (100 μM; 1h) induces microtubule stabilization in SW480 colon carcinoma cells[1].
IMM-01 (10 μM) significantly induces LacZ expression in NIH 3T3-SRE-LacZ cells[1].
IMM-01 (1-100 μM; 48 hours) induces caspase-3 cleavage during induction of apoptosis in NIH 3T3 cells and SW480 cells[1].
IMM-01 (5-25 mg/kg; i.v.; 2 times a week; for 3 weeks) is able to slow tumor growth in a mouse xenograft model of colon cancer[1].

Reference

[1]. L Leanne Lash, et al. Small-molecule intramimics of formin autoinhibition: a new strategy to target the cytoskeletal remodeling machinery in cancer cells. Cancer Res. 2013 Nov 15;73(22):6793-803.
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