Isopropyl ß-D-Thiogalactopyranoside, Dioxane Free

• CAT Number: R000145
• CAS Number: 367-93-1
• Molecular Formula: C9H18O5S
• Molecular Weight: 238.3
• Purity: ≥95%
• Synonyms: IPTG
• Storage: -20°C
• IUPAC Name: (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-propan-2-ylsulfanyloxane-3,4,5-triol
• InChI: InChI=1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• InChIKey: BPHPUYQFMNQIOC-NXRLNHOXSA-N
• SMILES: CC(C)S[C@H]1[C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O

Isopropyl β-D-thiogalactopyranoside, dioxane-free(Cat No.: R000145), is a synthetic, non-metabolizable analog of lactose used primarily in molecular biology to induce gene expression in systems regulated by the lac operon. It binds to the lac repressor, allowing transcription of downstream genes, often used in protein expression systems like E. coli. Being dioxane-free improves safety and purity for sensitive biological applications. IPTG is stable, highly effective at low concentrations, and essential in recombinant protein production and gene regulation studies.

Reference

Chang, Lewis M., and N. C. Horton. /Crystal Structure of the Lactose Operon Repressor and Its Complexes with DNA and Inducer./ Europe PubMed Central 271.5253 (1996): 1247-254. Europepmc.org. Web. 4 Dec. 2012.