For research use only. Not for therapeutic Use.
ISX-9(CAT: I005001) is a small molecule known for inducing differentiation in adult neural stem cells. Operating through specific mechanisms, ISX-9 has been shown to trigger neurogenesis while inhibiting gliogenesis in adult rat hippocampal stem cells. This effect occurs and is orchestrated via a calcium-activated signaling pathway that relies on myocyte-enhancer factor 2 (MEF2)-dependent gene expression. Notably, administration of ISX-9 at 20 mg/kg for a duration of 12 days in mice has exhibited improvements in hippocampal function, manifested by enhanced spatial memory ability as observed in the Morris water maze test.
| Catalog Number | I005001 |
| CAS Number | 832115-62-5 |
| Synonyms | N-cyclopropyl-5-(thiophen-2-yl)isoxazole-3-carboxamide |
| Molecular Formula | C11H10N2O2S |
| Purity | ≥95% |
| Target | Neuronal Signaling |
| Solubility | DMSO: ≥ 37 mg/mL |
| Storage | Store at +4C |
| InChI | InChI=1S/C11H10N2O2S/c14-11(12-7-3-4-7)8-6-9(15-13-8)10-2-1-5-16-10/h1-2,5-7H,3-4H2,(H,12,14) |
| InChIKey | SYENTKHGMVKGAQ-UHFFFAOYSA-N |
| SMILES | O=C(NC1CC1)C2=NOC(C3=CC=CS3)=C2 |
| Reference | 1:Neuroscience. 2016 Sep 22;332:212-22. doi: 10.1016/j.neuroscience.2016.06.042. Epub 2016 Jun 29. ISX-9 can potentiate cell proliferation and neuronal commitment in the rat dentate gyrus.Bettio LE,Patten AR,Gil-Mohapel J,O/’Rourke NF,Hanley RP,Kennedy S,Gopalakrishnan K,Rodrigues AL,Wulff J,Christie BR, PMID: 27373772 DOI: 10.1016/j.neuroscience.2016.06.042 </br><span>Abstract:</span> Adult hippocampal neurogenesis can be modulated by various physiological and pathological conditions, including stress, affective disorders, and several neurological conditions. Given the proposed role of this form of structural plasticity in the functioning of the hippocampus (namely learning and memory and affective behaviors), it is believed that alterations in hippocampal neurogenesis might underlie some of the behavioral deficits associated with these psychiatric and neurological conditions. Thus, the search for compounds that can reverse these deficits with minimal side effects has become a recognized priority. In the present study we tested the pro-neurogenic effects of isoxazole 9 (Isx-9), a small synthetic molecule that has been recently identified through the screening of chemical libraries in stem cell-based assays. We found that administration of Isx-9 for 14days was able to potentiate cell proliferation and increase the number of immature neurons in the hippocampal DG of adult rats. In addition, Isx-9 treatment was able to completely reverse the marked reduction in these initial stages of the neurogenic process observed in vehicle-treated animals (which were submitted to repeated handling and exposure to daily intraperitoneal injections). Based on these results, we recommend that future neurogenesis studies that require repeated handling and manipulation of animals should include a naïve (non-manipulated) control to determine the baseline levels of hippocampal cell proliferation and neuronal differentiation. Overall, these findings demonstrate that Isx-9 is a promising synthetic compound for the mitigation of stress-induced deficits in adult hippocampal neurogenesis. Future studies are thus warranted to evaluate the pro-neurogenic properties of Isx-9 in animal models of affective and neurological disorders associated with impaired hippocampal structural plasticity. Copyright © 2016 IBRO. Published by Elsevier Ltd. All rights reserved. |
