For research use only. Not for therapeutic Use.
JAK2 JH2 Tracer (Tracer 5) is a fluorescent probe for JAK2 JH2 domain, with a Kd of value 0.2 μM[1][2].
JAK2 JH2 Tracer shows a minimum tracer concentration that retains a satisfactory signal-to-noise ratio is found to be 1.5 pM[1].?
JAK2 JH2 Tracer shows a dissociation constants near 0.2 μM[1].?
JAK2 JH2 Tracer shows a high binding affinity with a Kd value of 0.2 μM for JAK2 JH2 domain by saturation experiments[1].
The possibility that mutations within the JAK2 JH2 domain may be associated with pathology, which is associated with a hyperactive kinase and a hematopoietic malignancy phenotype in flies[2].?
Specific mutation in the JAK2 JH2 domain is associated with several myeloproliferative diseases[2].
| Catalog Number | I013209 |
| CAS Number | 2101955-00-2 |
| Synonyms | 1-[[4-[[5-amino-1-(2,6-difluorobenzoyl)-1,2,4-triazol-3-yl]amino]phenyl]methyl]-3-(3′,6′-dihydroxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-yl)-1-methylthiourea |
| Molecular Formula | C38H27F2N7O6S |
| Purity | ≥95% |
| InChI | InChI=1S/C38H27F2N7O6S/c1-46(18-19-5-7-20(8-6-19)42-36-44-35(41)47(45-36)33(50)32-28(39)3-2-4-29(32)40)37(54)43-21-9-12-25-24(15-21)34(51)53-38(25)26-13-10-22(48)16-30(26)52-31-17-23(49)11-14-27(31)38/h2-17,48-49H,18H2,1H3,(H,43,54)(H3,41,42,44,45) |
| InChIKey | OAJQEPALUDJGLJ-UHFFFAOYSA-N |
| SMILES | CN(CC1=CC=C(C=C1)NC2=NN(C(=N2)N)C(=O)C3=C(C=CC=C3F)F)C(=S)NC4=CC5=C(C=C4)C6(C7=C(C=C(C=C7)O)OC8=C6C=CC(=C8)O)OC5=O |
| Reference | [1]. Newton AS, et al. JAK2 JH2 Fluorescence Polarization Assay and Crystal Structures for Complexes with Three Small Molecules. ACS Med Chem Lett. 2017 May 17;8(6):614-617. [2]. Ihle JN, Gilliland DG. Jak2: normal function and role in hematopoietic disorders. Curr Opin Genet Dev. 2007 Feb;17(1):8-14. |
