For research use only. Not for therapeutic Use.
Janelia Fluor 646, Azide (JF646, Azide) is a red fluorogenic fluorescent dye containing a click chemistry group Azide. Janelia Fluor 646, Azide can be used for live-cell imaging experiments[1][2]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Janelia Fluor? 646, Azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.
Maximum absorption wavelength (λabs)= 646 nm, maximum emission wavelength (λem)=664 nm[1].
JF646 can serve as a ligand for self-labeling tag (such as HaloTag)[1].
Janelia Fluor 646, Azide is modified by JF646. JF646, a red fluorescent dye, is photostable, membrane-permeable, has a high extinction coefficient[2].
| Catalog Number | I045771 |
| Synonyms | 2-[3-(azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethylbenzo[b][1]benzosilin-10-yl]-4-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethylcarbamoyl]benzoate |
| Molecular Formula | C37H44N6O6Si |
| Purity | ≥95% |
| InChI | InChI=1S/C37H44N6O6Si/c1-50(2)33-24-27(42-13-3-14-42)6-9-30(33)35(31-10-7-28(25-34(31)50)43-15-4-16-43)32-23-26(5-8-29(32)37(45)46)36(44)39-11-17-47-19-21-49-22-20-48-18-12-40-41-38/h5-10,23-25H,3-4,11-22H2,1-2H3,(H-,39,44,45,46) |
| InChIKey | FUJDWZJKTJZMHX-UHFFFAOYSA-N |
| SMILES | C[Si]1(C2=CC(=[N+]3CCC3)C=CC2=C(C4=C1C=C(C=C4)N5CCC5)C6=C(C=CC(=C6)C(=O)NCCOCCOCCOCCN=[N+]=[N-])C(=O)[O-])C |
| Reference | [1]. Grimm JB, Muthusamy AK, Liang Y, et al. A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat Methods. 2017;14(10):987-994. doi:10.1038/nmeth.4403. [2]. Basu S, et al. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nat Commun. 2018;9(1):2520. Published 2018 Jun 28. |
