For research use only. Not for therapeutic Use.
Janelia Fluor 646, Tetrazine (JF646, Tetrazine) a red fluorescent dye that contains a tetrazine group. JF646, Tetrazine can be used in cellular imaging[1]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Janelia Fluor? 646, Tetrazine is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.
Excitation maximum=646 nm; emission maximum=664 nm[1].
JF646 can serve as ligand for self-labeling tag (such as HaloTag) [1].
JF646, Tetrazine is modified by JF646. JF646, a red fluorescent dye, is photostable, membrane-permeable, has a high extinction coefficient[2]
| Catalog Number | I044580 |
| CAS Number | 2042192-00-5 |
| Synonyms | 2-[3-(azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethylbenzo[b][1]benzosilin-10-yl]-4-[[4-(1,2,4,5-tetrazin-3-yl)phenyl]methylcarbamoyl]benzoate |
| Molecular Formula | C38H35N7O3Si |
| Purity | ≥95% |
| InChI | InChI=1S/C38H35N7O3Si/c1-49(2)33-20-27(44-15-3-16-44)10-13-30(33)35(31-14-11-28(21-34(31)49)45-17-4-18-45)32-19-26(9-12-29(32)38(47)48)37(46)39-22-24-5-7-25(8-6-24)36-42-40-23-41-43-36/h5-14,19-21,23H,3-4,15-18,22H2,1-2H3,(H-,39,46,47,48) |
| InChIKey | CPWVRCGDSYQFSV-UHFFFAOYSA-N |
| SMILES | C[Si]1(C2=CC(=[N+]3CCC3)C=CC2=C(C4=C1C=C(C=C4)N5CCC5)C6=C(C=CC(=C6)C(=O)NCC7=CC=C(C=C7)C8=NN=CN=N8)C(=O)[O-])C |
| Reference | [1]. Grimm JB, et al. A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat Methods. 2015;12(3):244-250. doi:10.1038/nmeth.3256 [2]. Basu S, et al. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nat Commun. 2018;9(1):2520. Published 2018 Jun 28. |
