For research use only. Not for therapeutic Use.
Janelia Fluor 669, SE (JF669, SE), a red fluorescent dye, can be directly reacted with the available thiol-containing HaloTag ligand under mild conditions (DIEA, DMF) to afford a JF669HaloTag ligand in a single step (Ex = 669 nm; Em = 682 nm)[1]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
JF669 undergoes rapid conversion to thioether product (<5 min to completion) in organic solvents, where the dye predominantly adopts the closed form[1].
JF669 displays comparatively excellent photostability, retaining 97% fluorescence after the same number of cycles[1].
| Catalog Number | I014778 |
| CAS Number | 2127150-20-1 |
| Synonyms | 2-[3-(azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethylbenzo[b][1]benzosilin-10-yl]-4-[2-(2,5-dioxopyrrolidin-1-yl)oxy-2-oxoethyl]sulfanyl-3,5,6-trifluorobenzoate |
| Molecular Formula | C34H30F3N3O6SSi |
| Purity | ≥95% |
| InChI | InChI=1S/C34H30F3N3O6SSi/c1-48(2)22-15-18(38-11-3-12-38)5-7-20(22)27(21-8-6-19(16-23(21)48)39-13-4-14-39)28-29(34(44)45)30(35)32(37)33(31(28)36)47-17-26(43)46-40-24(41)9-10-25(40)42/h5-8,15-16H,3-4,9-14,17H2,1-2H3 |
| InChIKey | QFHKALIQFJQJKE-UHFFFAOYSA-N |
| SMILES | C[Si]1(C2=CC(=[N+]3CCC3)C=CC2=C(C4=C1C=C(C=C4)N5CCC5)C6=C(C(=C(C(=C6F)SCC(=O)ON7C(=O)CCC7=O)F)F)C(=O)[O-])C |
| Reference | [1]. Jonathan B Grimm, et al. General Synthetic Method for Si-Fluoresceins and Si-Rhodamines. ACS Cent Sci. 2017 Sep 27;3(9):975-985. |
