JKE-1674

• CAT Number: I029796
• CAS Number: 2421119-60-8
• Molecular Formula: C20H20Cl2N4O4
• Molecular Weight: 451.30
• Purity: 98%
• Synonyms: JKE-1674, JKE1674, JKE 1674
• Solubility: Soluble in DMSO
• Appearance: Solid powder
• Storage: 4°C, protect from light
• IUPAC Name: (2E)-1-[4-[bis(4-chlorophenyl)methyl]piperazin-1-yl]-2-hydroxyimino-3-nitropropan-1-one
• InChI: InChI=1S/C20H20Cl2N4O4/c21-16-5-1-14(2-6-16)19(15-3-7-17(22)8-4-15)24-9-11-25(12-10-24)20(27)18(23-28)13-26(29)30/h1-8,19,28H,9-13H2/b23-18+
• InChIKey: XIOHKIAPXVDWCP-PTGBLXJZSA-N
• SMILES: C1CN(CCN1C(C2=CC=C(C=C2)Cl)C3=CC=C(C=C3)Cl)C(=O)/C(=N/O)/C[N+](=O)[O-]

JKE-1674（Cat No.:I029796）is an inhibitor of glutathione peroxidase 4 (GPX4) and serves as an active metabolite of the GPX4 inhibitor ML-210. By targeting GPX4, JKE-1674 demonstrates the ability to reduce the viability of LOX-IMVI cancer cells and various other cancer cell lines. The observed effect can be counteracted by the ferroptosis inhibitor ferrostatin-1, indicating that JKE-1674 induces cell death through ferroptosis, a form of regulated cell death characterized by iron-dependent lipid peroxidation. These findings highlight the potential of JKE-1674 as a therapeutic strategy for targeting cancer cells via ferroptosis.

Reference

1. Eaton, J.K., Furst, L., Ruberto, R.A., et al. Selective covalent targeting of GPX4 using masked nitrile-oxide electrophiles. Nat. Chem. Biol. 16(5), 497-506 (2020).