KR-33494

• CAT Number: I011867
• CAS Number: 1021497-97-1
• Molecular Formula: C20H18BrN3O3S
• Molecular Weight: 460.3
• Purity: ≥95%
• Synonyms: 4-[[2-[(4-bromophenyl)thio]acetyl]amino]-1-(2-phenylethyl)-1H-pyrazole-3-carboxylic acid
• Target: FAF1
• Storage: -20°C
• InChI: InChI=1S/C20H18BrN3O3S/c21-15-6-8-16(9-7-15)28-13-18(25)22-17-12-24(23-19(17)20(26)27)11-10-14-4-2-1-3-5-14/h1-9,12H,10-11,13H2,(H,22,25)(H,26,27)
• InChIKey: ZRLJEHIUGYTTSZ-UHFFFAOYSA-N
• SMILES: BrC1=CC=C(SCC(NC2=CN(CCC3=CC=CC=C3)N=C2C(O)=O)=O)C=C1

KR-33494 (CAT: I011867) is a compound that serves as a fluorescent-tagged and biotin-tagged probe for Fas-associated factor 1 (FAF1). FAF1 is a protein involved in Fas-mediated cell death, which is a process of programmed cell death (apoptosis) triggered by the binding of the Fas ligand to the Fas receptor. The use of KR-33494 as a probe allows for the visualization and detection of FAF1 and its interactions within cellular processes related to Fas-mediated cell death. This type of probe can be valuable in studying the mechanisms and regulation of apoptosis and potentially aid in the development of therapeutic strategies targeting Fas signaling pathways.

Reference

1. Bioorg Med Chem Lett. 2016 Feb 15;26(4):1169-72. doi: 10.1016/j.bmcl.2016.01.045.
Epub 2016 Jan 18.
Design and synthesis of fluorescent and biotin tagged probes for the study of
molecular actions of FAF1 inhibitor.
Yoo SE(1), Yu C(2), Jung S(1), Kim E(2), Kang NS(1).
Author information:
(1)Graduate School of New Drug Discovery and Development, Chungnam National
University, Daehakno 99, Yuseong-gu, Daejeon 305-764, Republic of Korea.
(2)Department of Bioscience and Biotechnology, Chungnam National University,
Daehakno 99, Yuseong-gu, Daejeon 305-764, Republic of Korea.
To study the molecular action of ischemic Fas-mediated cell death inhibitor, we
prepared fluorescent-tagged and biotin-tagged probes of the potent inhibitor,
KR-33494, of ischemic cell death. We used the molecular modeling technique to
find the proper position for attaching those probes with minimum interference in
the binding process of probes with Fas-mediated cell death target, FAF1.