L-Lactate dehydrogenase, Microorganism

• CAT Number: I042549
• CAS Number: 9001-60-9
• Purity: ≥95%

L-Lactate dehydrogenase, Microorganism (LAD) is a redox enzyme. L-Lactate dehydrogenase catalyzes the reduction of pyruvate to L-lactate by NADH in vivo with absolute enantiospecificity[1].
Reaction conditions
Molecular weight:38 kDa (SDS-PAGE)
Isoelectric point:6.2
Optimum pH:6.5
Optimum temperature:45 ℃
pH Stability:4.5-10.0 (37℃，1h)
Thermal stability:<50 ℃ (pH7.4,15min） Inhibitors:Co2+，Cu2+，Fe3+，Ni2+，Zn2+，NEM，SDS，Proclin Protocol The enzyme dissolved in water

Reference

[1]. Simon ES, et al. D-lactate dehydrogenase. Substrate specificity and use as a catalyst in the synthesis of homochiral 2-hydroxy acids. Appl Biochem Biotechnol. 1989 Nov;22(2):169-79.
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[2]. Holmberg N, et al. Redesign of the coenzyme specificity in L-lactate dehydrogenase from bacillus stearothermophilus using site-directed mutagenesis and media engineering. Protein Eng. 1999 Oct;12(10):851-6.
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