L-Leucine-p-nitroanilide

• CAT Number: I014448
• CAS Number: 4178-93-2
• Molecular Formula: C12H17N3O3
• Molecular Weight: 251.28
• Purity: ≥95%
• Synonyms: L-Leucine-p-nitroanilide;;(S)-2-amino-4-methyl-N-(4-nitrophenyl)pentanamide
• Solubility: Soluble in DMSO
• InChI: InChI=1S/C12H17N3O3/c1-8(2)7-11(13)12(16)14-9-3-5-10(6-4-9)15(17)18/h3-6,8,11H,7,13H2,1-2H3,(H,14,16)/t11-/m0/s1
• InChIKey: AXZJHDNQDSVIDR-NSHDSACASA-N
• SMILES: CC(C)C[C@H](N)C(NC1=CC=C([N+]([O-])=O)C=C1)=O

L-Leucine-p-nitroanilide（Cat No.:I014448） is a substrate commonly used in the colorimetric determination of leucine aminopeptidase activity. Leucine aminopeptidase is an enzyme that cleaves the amino acid leucine from the N-terminus of peptides or proteins. When L-Leucine-p-nitroanilide is acted upon by leucine aminopeptidase, the p-nitroanilide group is released, resulting in a color change. This color change can be measured spectrophotometrically, providing a quantitative assessment of leucine aminopeptidase activity. L-Leucine-p-nitroanilide serves as a reliable and convenient substrate for studying and quantifying the enzymatic activity of leucine aminopeptidase.