For research use only. Not for therapeutic Use.
L-Proline p-nitroanilide TFA(CAT: M013677) is a synthetic amino acid derivative featuring a p-nitroanilide group and a trifluoroacetate (TFA) salt form, commonly used as a chromogenic substrate in enzymatic activity assays. It serves as a specific probe for studying protease activity, particularly proline-specific peptidases. Upon enzymatic cleavage, it releases p-nitroaniline, which can be quantitatively measured via spectrophotometry. This compound is widely utilized in biochemical and pharmaceutical research for enzyme kinetics, inhibitor screening, and assay development.
| CAS Number | 108321-19-3 |
| Synonyms | (2S)-N-(4-nitrophenyl)pyrrolidine-2-carboxamide;2,2,2-trifluoroacetic acid |
| Molecular Formula | C13H14F3N3O5 |
| Purity | ≥95% |
| IUPAC Name | (2S)-N-(4-nitrophenyl)pyrrolidine-2-carboxamide;2,2,2-trifluoroacetic acid |
| InChI | InChI=1S/C11H13N3O3.C2HF3O2/c15-11(10-2-1-7-12-10)13-8-3-5-9(6-4-8)14(16)17;3-2(4,5)1(6)7/h3-6,10,12H,1-2,7H2,(H,13,15);(H,6,7)/t10-;/m0./s1 |
| InChIKey | KYRVEVYREUUAKH-PPHPATTJSA-N |
| SMILES | C1CC(NC1)C(=O)NC2=CC=C(C=C2)[N+](=O)[O-].C(=O)(C(F)(F)F)O |
| Chemistry Calculators | Dilution Calculator In vivo Formulation Calculator Molarity Calculator Molecular Weight Calculator Reconstitution Calculator |
