For research use only. Not for therapeutic Use.
L82-G17 is an uncompetitive DNA ligase I (Lig I)-selective inhibitor. L82-G17 inhibits the third step of the ligation reaction, phosphodiester bond formation. L82-G17can be used as a probe of the catalytic activity[1].
L82-G17 (200 μM, 30 min) has selective uncompetitive inhibitory effect for LigI[1].
L82-G17 (0-100 μM) increases LigI binding to non-ligatable nicked DNA binding[1].
L82-G17 inhibits step 3 of the ligation reaction, phosphodiester bond formation[1].
L82-G17 (0-100 μM) inhibits DNA synthesis, cell viability and s induces DNA damage[1].
| Catalog Number | I030400 |
| CAS Number | 92285-87-5 |
| Synonyms | 5-chloro-4-[(2E)-2-[(3-hydroxyphenyl)methylidene]hydrazinyl]-1H-pyridazin-6-one |
| Molecular Formula | C11H9ClN4O2 |
| Purity | ≥95% |
| InChI | InChI=1S/C11H9ClN4O2/c12-10-9(6-14-16-11(10)18)15-13-5-7-2-1-3-8(17)4-7/h1-6,17H,(H2,15,16,18)/b13-5+ |
| InChIKey | PYYWVUBALUMAIY-WLRTZDKTSA-N |
| SMILES | C1=CC(=CC(=C1)O)C=NNC2=C(C(=O)NN=C2)Cl |
| Reference | [1]. Timothy R L Howes, et al. Structure-activity relationships among DNA ligase inhibitors: Characterization of a selective uncompetitive DNA ligase I inhibitor. DNA Repair (Amst). 2017 Dec;60:29-39. |
