Laurdan

• CAT Number: R066623
• CAS Number: 74515-25-6
• Molecular Formula: C24H35NO
• Molecular Weight: 353.55
• Purity: ≥95%
• Synonyms: 1-[6-(dimethylamino)-2-naphthalenyl]-1-dodecanone
• Storage: -20°C
• IUPAC Name: 1-[6-(dimethylamino)naphthalen-2-yl]dodecan-1-one
• InChI: InChI=1S/C24H35NO/c1-4-5-6-7-8-9-10-11-12-13-24(26)22-15-14-21-19-23(25(2)3)17-16-20(21)18-22/h14-19H,4-13H2,1-3H3
• InChIKey: JHDGGIDITFLRJY-UHFFFAOYSA-N
• SMILES: CCCCCCCCCCCC(=O)C1=CC2=C(C=C1)C=C(C=C2)N(C)C

Laurdan is a membrane permeable fluorescent probe that displays spectral sensitivity to the phospholipid phase of the cell membrane to which it is bound. Quantitation of generalize polarization (GP) of laurdan can be used to identify phospholipid phase. When excited at 340 nm, GP values are 0.6 and -0.2 for gel phase and liquid crystalline phase, respectively. GP does not change with polar head group or pH (in the range 4-10); it changes only with phase state.

Reference

1.Parasassi, T.,De Stasio, G.,Ravagnan, G., et al. Quantitation of lipid phases in phospholipid vesicles by the generalized polarization of Laurdan fluorescence. Biophys. J. 60(1), 179-189 (1991).