Lemobiline

• CAT Number: I030631
• CAS Number: 26126-76-1
• Molecular Formula: C15H17NO2
• Molecular Weight: 243.3
• Purity: 98%
• Synonyms: Lemobiline; Spectabiline; Lemobilin
• Solubility: Soluble in DMSO
• Appearance: Solid powder
• Storage: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
• IUPAC Name: 2,2,3,9-tetramethyl-3,9-dihydrofuro[2,3-b]quinolin-4(2H)-one
• InChI: InChI=1S/C15H17NO2/c1-9-12-13(17)10-7-5-6-8-11(10)16(4)14(12)18-15(9,2)3/h5-9H,1-4H3
• InChIKey: BXTFNWWMVUHWQA-UHFFFAOYSA-N
• SMILES: O=C1C2=C(OC(C)(C)C2C)N(C)C3=C1C=CC=C3

Lemobiline is a bioactive chemical.

Reference

1: Lange S, Rosenkrands I, Stein R, Andersen P, Kaufmann SH, Jungblut PR. Analysis of protein species differentiation among mycobacterial low-Mr-secreted proteins by narrow pH range Immobiline gel 2-DE-MALDI-MS. J Proteomics. 2014 Jan 31;97:235-44. doi: 10.1016/j.jprot.2013.06.036. Epub 2013 Jul 13. PubMed PMID: 23856608.
2: Fawcett JS, Vicchio D, Chrambach A. The adsorption of large proteins in electrofocusing on immobilized pH gradients: II. Dependence on the oligomeric state of Immobiline. Electrophoresis. 1988 Sep;9(9):469-74. PubMed PMID: 3243244.
3: Cherkaoui S, Zell P, Javet P. Characterization of immobiline membranes for application in a multicompartment electrolyzer for protein purification. J Biochem Biophys Methods. 1992 Aug;25(1):61-73. PubMed PMID: 1430792.
4: Chan C, Warlow RS, Chapuis PH, Newland RC, Bokey EL. Immobiline-based two-dimensional gel electrophoresis: an optimised protocol for resolution of human colonic mucosal proteins. Electrophoresis. 1999 Nov;20(17):3467-71. PubMed PMID: 10608716.
5: Bordini E, Hamdan M, Righetti PG. Assessment of the kinetics and sites of reaction of some immobiline chemicals with proteins and peptides by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2000;14(13):1141-8. PubMed PMID: 10867690.
6: Chiari M, Righetti PG. The Immobiline family: from "vacuum" to "plenum" chemistry. Electrophoresis. 1992 Apr;13(4):187-91. PubMed PMID: 1628597.
7: Tonani C, Faupel M, Righetti PG. Isoelectric membrane simulator: a computational approach for isoelectric immobiline membranes. Electrophoresis. 1991 Sep;12(9):631-6. PubMed PMID: 1752243.
8: Evans CH, Wilson AC, Gelléri BA. Preparative isoelectric focusing in ampholine electrofocusing columns versus immobiline polyacrylamide gel for the purification of biologically active leukoregulin. Anal Biochem. 1989 Mar;177(2):358-63. PubMed PMID: 2729555.
9: Fawcett JS, Chrambach A. The adsorption of large proteins in electrofocusing on immobilized pH gradients: I. Protein specificity and dependence on Immobiline and carrier ampholyte concentrations. Electrophoresis. 1988 Sep;9(9):463-9. PubMed PMID: 3243243.
10: Bini L, Sanchez-Campillo M, Santucci A, Magi B, Marzocchi B, Comanducci M, Christiansen G, Birkelund S, Cevenini R, Vretou E, Ratti G, Pallini V. Mapping of Chlamydia trachomatis proteins by immobiline-polyacrylamide two-dimensional electrophoresis: spot identification by N-terminal sequencing and immunoblotting. Electrophoresis. 1996 Jan;17(1):185-90. PubMed PMID: 8907538.
11: Bruschi M, Seppi C, Arena S, Musante L, Santucci L, Balduini C, Scaloni A, Lanciotti M, Righetti PG, Candiano G. Proteomic analysis of erythrocyte membranes by soft Immobiline gels combined with differential protein extraction. J Proteome Res. 2005 Jul-Aug;4(4):1304-9. PubMed PMID: 16083280.
12: Ettori C, Righetti PG, Comninellis C. Ti/IrO2 as anode and Zr as cathode in multicompartment electrolyzers with Immobiline membranes. Electrophoresis. 1992 Jan-Feb;13(1-2):55-8. PubMed PMID: 1587255.
13: Wenger P, de Zuanni M, Javet P, Gelfi C, Righetti PG. Amphoteric, isoelectric immobiline membranes for preparative isoelectric focusing. J Biochem Biophys Methods. 1987 Jan;14(1):29-43. PubMed PMID: 3693792.
14: Rabilloud T, Barzaghi B, Righetti PG. Use of polybuffer as carrier ampholytes in mixed-bed Immobiline gels for isoelectric focusing. J Biochem Biophys Methods. 1988 Jun-Jul;16(2-3):237-41. PubMed PMID: 3411086.
15: Görg A, Postel W, Günther S, Weser J, Strahler JR, Hanash SM, Somerlot L, Kuick R. Approach to stationary two-dimensional pattern: influence of focusing time and immobiline/carrier ampholytes concentrations. Electrophoresis. 1988 Jan;9(1):37-46. PubMed PMID: 2466646.
16: Sabounchi-Schütt F, Aström J, Olsson I, Eklund A, Grunewald J, Bjellqvist B. An immobiline DryStrip application method enabling high-capacity two-dimensional gel electrophoresis. Electrophoresis. 2000 Nov;21(17):3649-56. PubMed PMID: 11271483.
17: Holmquist L. Microheterogeneity of apolipoprotein D as revealed by electroblotting following isoelectric focusing in Immobiline DryPlates. Electrophoresis. 1992 Apr;13(4):262-4. PubMed PMID: 1628607.
18: Stoyanov AV, Righetti PG. pH changes in Immobiline gels due to low-molecular mass ion adsorption and conditions for salt front formation during electrophoretic desorption. Electrophoresis. 1997 Mar-Apr;18(3-4):344-8. PubMed PMID: 9150912.
19: Gåveby BM, Pettersson P, Andrasko J, Ineva-Flygare L, Johannesson U, Görg A, Postel W, Domscheit A, Mauri PL, Pietta P, et al. Stable storage conditions of Immobiline chemicals for isoelectric focusing. J Biochem Biophys Methods. 1988 Jun-Jul;16(2-3):141-64. PubMed PMID: 3411080.
20: Righetti PG, Chiari M, Casale E, Chiesa C. Oxidation of alkaline immobiline buffers for isoelectric focusing in immobilized pH gradients. Appl Theor Electrophor. 1989;1(2):115-21. PubMed PMID: 2488590.