For research use only. Not for therapeutic Use.
Lucifer Yellow CH dipotassium is a high-intensity fluorescent probe containing free hydrazyl groups. Lucifer Yellow CH can react with fatty aldehydes at room temperature. Lucifer Yellow CH serves as a biological tracer to monitor neuronal branching, regeneration, gap junction detection and characterization, and selective ablation of cells after aldehyde fixation. Lucifer yellow CH displays the maximum excitation/emission of 430 nm/540 nm, respectively[1][2].
Preparation of Lucifer Yellow CH (dipotassium) working solution 1 Preparation of the stock solution Dissolve Lucifer Yellow CH (dipotassium) in DDH2O to obtain 1 mg/mL of Lucifer Yellow CH (dipotassium). Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.2. Preparation of Lucifer Yellow CH (dipotassium) working solution. Dilute the stock solution in serum-free cell culture medium or PBS to obtain 0.5% of Lucifer Yellow CH (dipotassium) working solution. Note: Please adjust the concentration of Lucifer Yellow CH (dipotassium) working solution according to the actual situation. Cell staining 1. Cell preparation:For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. 2. Add 1 mL of Lucifer Yellow CH (dipotassium) working solution, and then incubate at room temperature for 5-30 minutes. 3. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. 4. Wash twice with PBS, 5 minutes each time. 5. Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer. Precautions 1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles. 2. Please adjust the concentration of Lucifer Yellow CH (dipotassium) working solution according to the actual situation. 3. This product is for R&D use only, not for drug, household, or other uses. 4. For your safety and health, please wear a lab coat and disposable gloves to operate.
| Catalog Number | R072042 |
| CAS Number | 71206-95-6 |
| Synonyms | dipotassium;6-amino-2-(hydrazinecarbonylamino)-1,3-dioxobenzo[de]isoquinoline-5,8-disulfonate |
| Molecular Formula | C13H9K2N5O9S2 |
| Purity | ≥95% |
| InChI | InChI=1S/C13H11N5O9S2.2K/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2 |
| InChIKey | HCWYSCVNSCIZCN-UHFFFAOYSA-L |
| SMILES | C1=C(C=C2C3=C1C(=C(C=C3C(=O)N(C2=O)NC(=O)NN)S(=O)(=O)[O-])N)S(=O)(=O)[O-].[K+].[K+] |
| Reference | [1]. Klein M, et al. Transport of lucifer yellow CH into plant vacuoles–evidence for direct energization of a sulphonatedsubstance and implications for the design of new molecular probes. FEBS Lett. 1997 Dec 22;420(1):86-92. |
