Lumogallion

• CAT Number: I031128
• CAS Number: 4386-25-8
• Molecular Formula: C12H9ClN2O6S
• Molecular Weight: 344.73
• Purity: ≥95%
• Synonyms: 5-chloro-3-[(2,4-dihydroxyphenyl)diazenyl]-2-hydroxybenzenesulfonic acid
• InChI: InChI=1S/C12H9ClN2O6S/c13-6-3-9(12(18)11(4-6)22(19,20)21)15-14-8-2-1-7(16)5-10(8)17/h1-5,16-18H,(H,19,20,21)
• InChIKey: OTKYOGBGHUUFPC-UHFFFAOYSA-N
• SMILES: C1=CC(=C(C=C1O)O)N=NC2=C(C(=CC(=C2)Cl)S(=O)(=O)O)O

Lumogallion is a highly sensitive fluorescent reagent for the detection of aluminum, gallium and other metals. Lumogallion has an excitation wavelength of 490 nm and an emission spectrum in the range of 520 nm to 650 nm, with a peak near 580 nm[1][2].
Lumogallion (1 mM, 45 min) can be used to detect aluminum levels in brain tissue of Alzheimer’s patients[1].
Lumogallion can be used as a probe to stain live cells with intracellular aluminum adjuvant[2].
Lumogallion (10 μM, 60 min) can be used to detect aluminum content in root tip cell tissue of plants[3].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolved lumogallion in 0.1 M acetate buffer (pH 5.2).
2. Sample and 10 μM lumogallion were co-incubated for 60 min in the dark.
3. Washed the mixed sample well with acetate buffer solution twice, 15 minutes each, and observed under a confocal laser microscope.
4. Excitation wavelength is 488 nm , emission wavelength is 520 nm.

Reference

[1]. Ambreen Mirza, et al. The Identification of Aluminum in Human Brain Tissue Using Lumogallion and Fluorescence Microscopy. J Alzheimers Dis. 2016 Oct 18;54(4):1333-1338.
 [Content Brief]

[2]. Irene Mile, et al. Al adjuvants can be tracked in viable cells by lumogallion staining. J Immunol Methods. 2015 Jul;422:87-94.
 [Content Brief]

[3]. Tatsuhiko Kataoka, et al. Highly sensitive analytical method for aluminum movement in soybean root through lumogallion staining. J. Plant Res. 110, 305–309 (1997).