Lys-Pro-AMC diTFA

• CAT Number: I040713
• Molecular Formula: C25H30F6N4O8
• Molecular Weight: 628.52
• Purity: ≥95%
• Synonyms: (2S)-1-[(2S)-2,6-diaminohexanoyl]-N-(4-methyl-2-oxochromen-7-yl)pyrrolidine-2-carboxamide;2,2,2-trifluoroacetic acid
• InChI: InChI=1S/C21H28N4O4.2C2HF3O2/c1-13-11-19(26)29-18-12-14(7-8-15(13)18)24-20(27)17-6-4-10-25(17)21(28)16(23)5-2-3-9-22;2*3-2(4,5)1(6)7/h7-8,11-12,16-17H,2-6,9-10,22-23H2,1H3,(H,24,27);2*(H,6,7)/t16-,17-;;/m0../s1
• InChIKey: UYHIEPMMURECAA-SZKOKKDDSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C3CCCN3C(=O)C(CCCCN)N.C(=O)(C(F)(F)F)O.C(=O)(C(F)(F)F)O

Lys-Pro-AMC diTFA(CAT: I040713) is a fluorogenic peptide substrate composed of lysine and proline residues conjugated to 7-amino-4-methylcoumarin (AMC), with di-trifluoroacetate (diTFA) as the counterion. Upon enzymatic cleavage, AMC is released and emits a measurable fluorescent signal, making this compound ideal for monitoring protease activity, particularly prolyl-specific peptidases and lysine-targeting enzymes. It is widely used in enzyme kinetics, inhibitor screening, and biochemical assay development. The diTFA salt ensures improved solubility and stability for experimental applications.

Reference

[1]. Pavla Fajtová, et al. Prolyl Oligopeptidase from the Blood Fluke Schistosoma mansoni: From Functional Analysis to Anti-schistosomal Inhibitors. PLoS Negl Trop Dis. 2015 Jun 3;9(6):e0003827.
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