LysoTracker Yellow HCK 123

• CAT Number: I043052
• CAS Number: 1064123-31-4
• Molecular Formula: C16H24N6O4
• Molecular Weight: 364.40
• Purity: ≥95%
• Synonyms: N-[2-(dimethylamino)ethyl]-6-[(4-nitro-2,1,3-benzoxadiazol-7-yl)amino]hexanamide
• InChI: InChI=1S/C16H24N6O4/c1-21(2)11-10-18-14(23)6-4-3-5-9-17-12-7-8-13(22(24)25)16-15(12)19-26-20-16/h7-8,17H,3-6,9-11H2,1-2H3,(H,18,23)
• InChIKey: WIOHDRVBBRICQL-UHFFFAOYSA-N
• SMILES: CN(C)CCNC(=O)CCCCCNC1=CC=C(C2=NON=C12)[N+](=O)[O-]

LysoTracker Yellow HCK 123 is a potent yellow membrane-permeable fluorescent probe. LysoTracker Yellow HCK 123 is a weakly basic amine that selectively accumulates in cellular compartments with low luminal pH. (λex=465 nm, λem=535 nm)[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
LysoTracker Yellow HCK 123 assay[1] (to quantify GAPDH):
1. Culture EMT6 cells in 8-well cell culture slides. Incubate the cells according to your normal protocol.
2. Add TONS504 per well to make the final concentration at 30 µg/mL.Incubate cells at 37 °C for 4 hours.
3. Add LysoTracker Yellow HCK 123 per well to make the final concentration at 50 μM. Incubate cells at 37 °C, 5% CO2, for 30 minutes.
4. Analyze samples on a confocal laser scanning microscope.

Reference

[1]. Desclés J, et, al. New tools for labeling silica in living diatoms. New Phytol. 2008;177(3):822-829.
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