Malic dehydrogenase, microorganism

• CAT Number: I041901
• CAS Number: 9001-64-3
• Purity: ≥95%

Malate dehydrogenase (EC 1.1.1.37) (MDH) catalyzes the mutual conversion of oxaloacetate and malate, and is associated with the oxidation/reduction of dinucleotide coenzymes[1].
Molecular weight: approx: 35,000 (by gel filtration)
Michaelis constants: 1.5×10-6 M (NADH, 37°C),1.9×10-6 M (Oxaloacetate, 37°C)
Optimu M pH: 7.5-8.0
Optimu M temperature: 60°C
pH Stability: 3.0-9.0 (37°C, 30 min)
Thermal stability: below 65℃ (pH 7.5, 30 min)
Solubility: MDH was dissolved at concentration of 20 U/mL in 100 mM, pH 7.5,Tris-HCl buffer.

Reference

[1]. Goward CR, et al. Malate dehydrogenase: a model for structure, evolution, and catalysis. Protein Sci. 1994 Oct;3(10):1883-8.
 [Content Brief]