MCA-ASP-GLU-VAL-ASP-ALA-PRO-LYS(DNP)-OH

• CAT Number: M041739
• CAS Number: 189696-20-6
• Molecular Formula: C50H62N10O22
• Molecular Weight: 1155.09
• Purity: ≥95%
• Target: Caspase
• Storage: -20°C
• IUPAC Name: (2S)-2-[[(2S)-1-[(2S)-2-[[(2S)-3-carboxy-2-[[(2S)-2-[[(2S)-4-carboxy-2-[[(2S)-3-carboxy-2-[[2-(7-methoxy-2-oxochromen-4-yl)acetyl]amino]propanoyl]amino]butanoyl]amino]-3-methylbutanoyl]amino]propanoyl]amino]propanoyl]pyrrolidine-2-carbonyl]amino]-6-(2,4-dinitroanilino)hexanoic acid
• InChI: InChI=1S/C50H62N10O22/c1-24(2)43(57-44(69)31(14-15-39(62)63)54-46(71)33(22-40(64)65)53-38(61)18-26-19-42(68)82-37-21-28(81-4)11-12-29(26)37)48(73)56-34(23-41(66)67)45(70)52-25(3)49(74)58-17-7-9-35(58)47(72)55-32(50(75)76)8-5-6-16-51-30-13-10-27(59(77)78)20-36(30)60(79)80/h10-13,19-21,24-25,31-35,43,51H,5-9,14-18,22-23H2,1-4H3,(H,52,70)(H,53,61)(H,54,71)(H,55,72)(H,56,73)(H,57,69)(H,62,63)(H,64,65)(H,66,67)(H,75,76)/t25-,31-,32-,33-,34-,35-,43-/m0/s1
• InChIKey: YOUVSUDOGAVRQX-AEAFXXELSA-N
• SMILES: C[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCNC2=C(C=C(C=C2)[N+](=O)[O-])[N+](=O)[O-])C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)CC3=CC(=O)OC4=C3C=CC(=C4)OC

MCA-ASP-GLU-VAL-ASP-ALA-PRO-LYS(DNP)-OH（Cat No.:M041739）is a synthetic fluorogenic peptide substrate widely used in enzymology and protease activity studies, particularly for caspase assays. The MCA (7-methoxycoumarin-4-acetyl) group acts as a fluorophore, while the DNP (2,4-dinitrophenyl) group quenches fluorescence until proteolytic cleavage occurs. Upon cleavage by caspases, fluorescence is restored, allowing for real-time monitoring of enzyme activity. This substrate is valuable for studying apoptosis pathways, protease specificity, and inhibitor screening. Its sensitivity and versatility make it a critical tool in biochemical and pharmaceutical research.