Mca-YVADAP-Lys(Dnp)-OH TFA

• CAT Number: I040979
• Molecular Formula: C55H65F3N10O21
• Molecular Weight: 1259.15
• Purity: ≥95%
• Synonyms: (2S)-2-[[(2S)-1-[(2S)-2-[[(2S)-3-carboxy-2-[[(2S)-2-[[(2S)-2-[[(2S)-3-(4-hydroxyphenyl)-2-[[2-(7-methoxy-2-oxochromen-4-yl)acetyl]amino]propanoyl]amino]-3-methylbutanoyl]amino]propanoyl]amino]propanoyl]amino]propanoyl]pyrrolidine-2-carbonyl]amino]-6-(2,4-dinitroanilino)hexanoic acid;2,2,2-trifluoroacetic acid
• InChI: InChI=1S/C53H64N10O19.C2HF3O2/c1-27(2)46(60-49(71)38(21-30-11-14-33(64)15-12-30)57-43(65)22-31-23-45(68)82-42-25-34(81-5)16-17-35(31)42)51(73)55-28(3)47(69)59-39(26-44(66)67)48(70)56-29(4)52(74)61-20-8-10-40(61)50(72)58-37(53(75)76)9-6-7-19-54-36-18-13-32(62(77)78)24-41(36)63(79)80;3-2(4,5)1(6)7/h11-18,23-25,27-29,37-40,46,54,64H,6-10,19-22,26H2,1-5H3,(H,55,73)(H,56,70)(H,57,65)(H,58,72)(H,59,69)(H,60,71)(H,66,67)(H,75,76);(H,6,7)/t28-,29-,37-,38-,39-,40-,46-;/m0./s1
• InChIKey: IDHHNDRKPWTJSE-VXJHEXFUSA-N
• SMILES: C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCNC2=C(C=C(C=C2)[N+](=O)[O-])[N+](=O)[O-])C(=O)O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC3=CC=C(C=C3)O)NC(=O)CC4=CC(=O)OC5=C4C=CC(=C5)OC.C(=O)(C(F)(F)F)O

Mca-YVADAP-Lys(Dnp)-OH TFA(CAT: I040979) is a fluorogenic peptide substrate designed for caspase-1 (ICE, interleukin-1β converting enzyme) activity assays. It contains a 7-methoxycoumarin-4-acetyl (Mca) donor fluorophore and a 2,4-dinitrophenyl (Dnp) quencher, enabling fluorescence-based detection of caspase-mediated cleavage at the YVAD (Tyr-Val-Ala-Asp) recognition site. This substrate is widely used in in vitro enzyme kinetics, drug screening, and inflammation research, particularly in studies related to apoptosis, pyroptosis, and immune response modulation. The TFA salt form ensures stability and solubility for experimental applications. For research use only; not for human or clinical applications.