MeO-Suc-Arg-Pro-Tyr-pNA

• CAT Number: I040748
• CAS Number: 82564-18-9
• Molecular Formula: C31H40N8O9
• Molecular Weight: 668.70
• Purity: ≥95%
• Synonyms: methyl 4-[[(2S)-5-(diaminomethylideneamino)-1-[(2S)-2-[[(2S)-3-(4-hydroxyphenyl)-1-(4-nitroanilino)-1-oxopropan-2-yl]carbamoyl]pyrrolidin-1-yl]-1-oxopentan-2-yl]amino]-4-oxobutanoate
• InChI: InChI=1S/C31H40N8O9/c1-48-27(42)15-14-26(41)36-23(4-2-16-34-31(32)33)30(45)38-17-3-5-25(38)29(44)37-24(18-19-6-12-22(40)13-7-19)28(43)35-20-8-10-21(11-9-20)39(46)47/h6-13,23-25,40H,2-5,14-18H2,1H3,(H,35,43)(H,36,41)(H,37,44)(H4,32,33,34)/t23-,24-,25-/m0/s1
• InChIKey: TXYMHQOSDJXNHI-SDHOMARFSA-N
• SMILES: COC(=O)CCC(=O)NC(CCCN=C(N)N)C(=O)N1CCCC1C(=O)NC(CC2=CC=C(C=C2)O)C(=O)NC3=CC=C(C=C3)[N+](=O)[O-]

MeO-Suc-Arg-Pro-Tyr-pNA(CAT: I040748) is a synthetic chromogenic peptide substrate used for detecting and quantifying serine protease activity, particularly enzymes with chymotrypsin-like specificity. The MeO-Suc (methoxy-succinyl) group enhances stability and enzyme selectivity, while the Arg-Pro-Tyr sequence serves as a specific recognition motif. Upon cleavage by the target protease, the p-nitroanilide (pNA) moiety is released, producing a yellow color measurable at 405 nm. This substrate is widely used in biochemical assays, enzyme kinetics, and inhibitor screening. Its high sensitivity and compatibility with microplate formats make it ideal for high-throughput enzymatic studies in drug discovery and protease-related research.