meta-iodoHoechst 33258

• CAT Number: I002007
• CAS Number: 158013-42-4
• Molecular Formula: C25H23IN6
• Molecular Weight: 534.39
• Purity: ≥95%
• Synonyms: 2/’-(3-iodophenyl)-6-(4-methylpiperazin-1-yl)-1H,3/’H-2,5/’-bibenzo[d]imidazole
• Target: DNA Stain
• Solubility: DMSO or water Protect from light
• Storage: Store at -20°C

meta-iodoHoechst 33258 (CAT: I002007) is a member of the Hoechst family of blue fluorescent dyes commonly used for DNA staining. Like other Hoechst dyes, meta-iodoHoechst 33258 is excited by ultraviolet light at around 350 nm and emits blue/cyan fluorescent light around an emission maximum of 461 nm. These dyes specifically bind to the minor groove of double-stranded DNA, with a preference for sequences rich in adenine and thymine. They are cell-permeable and can be used to stain DNA in live or fixed cells. The fluorescence emitted by the bound dye allows for the visualization and quantification of DNA in various biological samples.

Reference

[1]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4/’,6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68.

[2]. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505.

[3]. a b c /Hoechst Stains/. Invitrogren (Molecular Probes).