For research use only. Not for therapeutic Use.
Mito-TRFS, the first off-on probe, is used to image the mitochondrial thioredoxin reductase (TrxR2) in live cells[1].
Mito-TRFS (10 μM) has the maximal absorption at ~375 nm in TE buffer (50 mM Tris-HCl and 1 mM EDTA, pH 7.4) at 37 °C[1].
Mito-TRFS itself has a strong emission at ~480 nm when excited at 375 nm in TE buffer (φ=0.34), but has weak emission when excited at 438 nm[1].
| Catalog Number | I032367 |
| CAS Number | 1859102-62-7 |
| Synonyms | 2-[2-[6-(dithiolan-4-yloxycarbonylamino)-1,3-dioxobenzo[de]isoquinolin-2-yl]ethoxy]ethyl-triphenylphosphanium;iodide |
| Molecular Formula | C38H34IN2O5PS2 |
| Purity | ≥95% |
| InChI | InChI=1S/C38H33N2O5PS2.HI/c41-36-32-18-10-17-31-34(39-38(43)45-27-25-47-48-26-27)20-19-33(35(31)32)37(42)40(36)21-22-44-23-24-46(28-11-4-1-5-12-28,29-13-6-2-7-14-29)30-15-8-3-9-16-30;/h1-20,27H,21-26H2;1H |
| InChIKey | UKWAKZCQJRRDFN-UHFFFAOYSA-N |
| SMILES | C1C(CSS1)OC(=O)NC2=C3C=CC=C4C3=C(C=C2)C(=O)N(C4=O)CCOCC[P+](C5=CC=CC=C5)(C6=CC=CC=C6)C7=CC=CC=C7.[I-] |
| Reference | [1]. Yaping Liu, et al. A small molecule probe reveals declined mitochondrial thioredoxin reductase activity in a Parkinson’s disease model. Chem Commun (Camb). 2016 Feb 7;52(11):2296-9. |
