MRT 10

• CAT Number: I010986
• CAS Number: 330829-30-6
• Molecular Formula: C24H23N3O5S
• Molecular Weight: 465.52
• Purity: ≥95%
• Synonyms: N-[[[3-Benzoylamino)phenyl]amino]thioxomethyl]-3,4,5-trimethoxybenzamide
• Target: Smo
• Solubility: Soluble to 100 mM in DMSO
• Storage: Store at +4C
• IUPAC Name: N-[(3-benzamidophenyl)carbamothioyl]-3,4,5-trimethoxybenzamide
• InChI: InChI=1S/C24H23N3O5S/c1-30-19-12-16(13-20(31-2)21(19)32-3)23(29)27-24(33)26-18-11-7-10-17(14-18)25-22(28)15-8-5-4-6-9-15/h4-14H,1-3H3,(H,25,28)(H2,26,27,29,33)
• InChIKey: KVQVEZQDNHMQJV-UHFFFAOYSA-N
• SMILES: COC1=CC(=CC(=C1OC)OC)C(=O)NC(=S)NC2=CC=CC(=C2)NC(=O)C3=CC=CC=C3

MRT 10(CAT: I010986) is a potent Smoothened (Smo) receptor antagonist with an IC 50 of 0.5 μM in HEK293 cells transiently expressing mouse Smo. It effectively inhibits Sonic Hedgehog (ShhN)-induced Gli luciferase reporter activity in Shh-light 2 cells with an IC50 of 2.5 μM. Additionally, it functions as an inverse agonist during nontranscriptional activation of the Hedgehog signaling pathway. MRT 10 is a valuable tool for studying Hedgehog pathway regulation and its implications in cancer and developmental disorders, offering insights into novel therapeutic strategies targeting Smo-dependent mechanisms.