Msr-blue

• CAT Number: I045002
• CAS Number: 2966537-39-1
• Molecular Formula: C11H10O3S
• Molecular Weight: 222.26
• Purity: ≥95%
• Synonyms: 4-methyl-7-methylsulfinylchromen-2-one
• InChI: InChI=1S/C11H10O3S/c1-7-5-11(12)14-10-6-8(15(2)13)3-4-9(7)10/h3-6H,1-2H3
• InChIKey: IFTHUDRVKVIXDN-UHFFFAOYSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)S(=O)C

Msr-blue is a first turn-on fluorescent probe for methionine sulfoxide reductase with a more than 100-fold fluorescence increment. Msr-blue is used for monitoring the enzyme activity in live cells (λex=340 nm, λem=440 nm)[1].
Msr-blue is emited blue fluorescence after activation by methionine sulfoxide reductase A (Msr A). Msr-blue responded to Msr A in both a time- and dose-dependent manner, and more than a 100-fold increase in the emission is observed. Msr-blue is converted to its corresponding sulfide (15′) under catalysis by either the purified Msr A or a cell lysate[1].
The 6-OHDA-treated PC12 cells as a cellular model of Parkinson’s disease (PD) is employed and applied Msr-blue to probe the function of Msrs in the cells. With the aid of Msr-blue, a decline of the Msr activity in a PD model was disclosed for the first time[1].

Reference

[1]. Liangwei Zhang, et al. A specific fluorescent probe reveals compromised activity of methionine sulfoxide reductases in Parkinson’s disease. Chem Sci. 2017 Apr 1;8(4):2966-2972.
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