Msr-Ratio

• CAT Number: I045001
• CAS Number: 2290635-22-0
• Molecular Formula: C18H14O3S
• Molecular Weight: 310.37
• Purity: ≥95%
• Synonyms: 2-[(E)-2-(4-methylsulfinylphenyl)ethenyl]chromen-4-one
• InChI: InChI=1S/C18H14O3S/c1-22(20)15-10-7-13(8-11-15)6-9-14-12-17(19)16-4-2-3-5-18(16)21-14/h2-12H,1H3/b9-6+
• InChIKey: ICJBRZQQFOCOJU-RMKNXTFCSA-N
• SMILES: CS(=O)C1=CC=C(C=C1)C=CC2=CC(=O)C3=CC=CC=C3O2

Msr-Ratio (Msr-green) is a ratiometric fluorescent probe of methionine sulfoxide reductase (λex=375 nm, λem=550 nm). Msr-Ratio is used for monitoring the enzyme activity in vitro and in live cells[1].
Msr-Ratio is converted to its corresponding sulfide form by methionine sulfoxide reductase. Msr-Ratio displays favorable properties such as a nearly 400-fold fluorescence change, fast response rate (<30 min), large Stokes shift (120 nm), and green emission (550 nm)[1]. There is a very weak background signal in live HL60 cells after the addition of Msr-Ratio. As the Msr-Ratio (10 μM) incubation time increased in cells from 0 to 8 h, the fluorescence signal constantly strengthened[1].

Reference

[1]. Liangwei Zhang, et al. A ratiometric fluorescent probe of methionine sulfoxide reductase with an improved response rate and emission wavelength. Chem Commun (Camb). 2019 Jan 29;55(10):1502-1505.
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