N-Hydroxysulfosuccinimide Sodium Salt

• CAT Number: R000135
• CAS Number: 106627-54-7
• Molecular Formula: C4H4NNaO6S
• Molecular Weight: 217.127
• Purity: ≥95%
• Synonyms: 1-Hydroxy-2,5-dioxo-3-pyrrolidinesulfonic Acid Sodium Salt; Sodium N-Hydroxysulfosuccinimide; Sulfo-N-hydroxysuccinimide Sodium Salt;?
• Target: ADC Linker
• Storage: -20°C
• IUPAC Name: sodium;1-hydroxy-2,5-dioxopyrrolidine-3-sulfonate
• InChI: InChI=1S/C4H5NO6S.Na/c6-3-1-2(12(9,10)11)4(7)5(3)8;/h2,8H,1H2,(H,9,10,11);/q;+1/p-1
• InChIKey: RPENMORRBUTCPR-UHFFFAOYSA-M
• SMILES: C1C(C(=O)N(C1=O)O)S(=O)(=O)[O-].[Na+]

N-Hydroxysulfosuccinimide sodium salt (Sulfo-NHS) is a water-soluble reagent commonly used in bioconjugation and protein labeling techniques. It is often paired with carbodiimides like EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) to activate carboxyl groups on biomolecules, facilitating their coupling to primary amines on proteins, peptides, or other molecules. The sulfonated nature of Sulfo-NHS enhances its solubility in aqueous solutions, making it particularly useful in biological applications. It is widely used in the preparation of antibody-drug conjugates, enzyme immobilization, and surface modification processes in molecular biology and bioengineering. Its high efficiency in crosslinking reactions makes it a valuable tool in biochemical research.