For research use only. Not for therapeutic Use.
Necrostatin 2(CAT: I005117) is a potent inhibitor of necroptosis, a regulated form of cell death that resembles necrosis. It has an IC50 value of 50 nM (EC50) and primarily targets TNF-alpha. Necrostatin 2 has demonstrated efficacy in inhibiting necroptosis both in vitro and in an animal model of ischemic stroke. It belongs to a class of compounds known as necrostatin derivatives, which have been designed as tools for researchers studying necroptosis. Necroptosis inhibitors, such as necrostatin 2, have shown promise in understanding the molecular mechanisms of necroptosis and may have therapeutic applications in diseases associated with this cell death pathway.
| Catalog Number | I005117 |
| CAS Number | 852391-19-6 |
| Synonyms | (5R)-5-[(7-chloro-1H-indol-3-yl)methyl]-3-methylimidazolidine-2,4-dione |
| Molecular Formula | C13H12ClN3O2 |
| Purity | ≥95% |
| Target | RIP kinase |
| Solubility | 10 mM in DMSO |
| Storage | Store at -20°C |
| IC50 | 50 nM (EC50) [1] |
| InChIKey | WIKGAEMMNQTUGL-SNVBAGLBSA-N |
| Reference | </br>1:Clathrin-dependent endocytosis of claudin-2 by DFYSP peptide causes lysosomal damage in lung adenocarcinoma A549 cells. Ikari A, Taga S, Watanabe R, Sato T, Shimobaba S, Sonoki H, Endo S, Matsunaga T, Sakai H, Yamaguchi M, Yamazaki Y, Sugatani J.Biochim Biophys Acta. 2015 Oct;1848(10 Pt A):2326-36. doi: 10.1016/j.bbamem.2015.07.003. Epub 2015 Jul 8. PMID: 26163137 Free Article</br>2:2-Phenylethynesulfonamide (PES) uncovers a necrotic process regulated by oxidative stress and p53. Mattiolo P, Barbero-Farran A, Yuste VJ, Boix J, Ribas J.Biochem Pharmacol. 2014 Oct 1;91(3):301-11. doi: 10.1016/j.bcp.2014.08.005. Epub 2014 Aug 16. PMID: 25139326 </br>3:Involvement of caspase-2 activation in aurora kinase inhibitor-induced cell death in axin-expressing L929 cells. Choi EJ, Kim SM, Shin JH, Kim S, Song KJ, Kee SH.Apoptosis. 2014 Apr;19(4):657-67. doi: 10.1007/s10495-013-0951-2. PMID: 24287941 </br>4:The predominant protective effect of tianeptine over other antidepressants in models of neuronal apoptosis: the effect blocked by inhibitors of MAPK/ERK1/2 and PI3-K/Akt pathways. Jantas D, Krawczyk S, Lason W.Neurotox Res. 2014 Feb;25(2):208-25. doi: 10.1007/s12640-013-9430-3. Epub 2013 Oct 9. PMID: 24105645 Free PMC Article</br>5:Sulphur antioxidants inhibit oxidative stress induced retinal ganglion cell death by scavenging reactive oxygen species but influence nuclear factor (erythroid-derived 2)-like 2 signalling pathway differently. Majid AS, Yin ZQ, Ji D.Biol Pharm Bull. 2013;36(7):1095-110. PMID: 23811559 Free Article</br>6:Necrosis-like death can engage multiple pro-apoptotic Bcl-2 protein family members. Tischner D, Manzl C, Soratroi C, Villunger A, Krumschnabel G.Apoptosis. 2012 Nov;17(11):1197-209. doi: 10.1007/s10495-012-0756-8. PMID: 22971741 Free PMC Article</br>7:Nec-1 enhances shikonin-induced apoptosis in leukemia cells by inhibition of RIP-1 and ERK1/2. Han W, Xie J, Fang Y, Wang Z, Pan H.Int J Mol Sci. 2012;13(6):7212-25. doi: 10.3390/ijms13067212. Epub 2012 Jun 12. PMID: 22837689 Free PMC Article</br>8:TNF-α/Fas-RIP-1-induced cell death signaling separates murine hematopoietic stem cells/progenitors into 2 distinct populations. Xiao Y, Li H, Zhang J, Volk A, Zhang S, Wei W, Zhang S, Breslin P, Zhang J.Blood. 2011 Dec 1;118(23):6057-67. doi: 10.1182/blood-2011-06-359448. Epub 2011 Oct 11. PMID: 21989986 Free Article</br>9:Necrostatin-1 ameliorates symptoms in R6/2 transgenic mouse model of Huntington/’s disease. Zhu S, Zhang Y, Bai G, Li H.Cell Death Dis. 2011;2:e115. doi: 10.1038/cddis.2010.94. No abstract available. PMID: 21359116 Free PMC Article |
