Neferine

• CAT Number: I002749
• CAS Number: 2292-16-2
• Molecular Formula: C38H44N2O6
• Molecular Weight: 624.77
• Purity: ≥95%
• Target: target: NF-κB [1]
• Solubility: DMSO ≥ 15 mg/mL
• Storage: -20°C

Neferine, is a major alkaloid present in the green embryos of Nelumbo nucifera Gaertn with anti-arrhythmia, anti-hypertensive and vaso-relaxant properties , also a NF-κB inhibitor.target: NF-κB [1]IC50: 1.25 uM ( neonatal rat CMs ) [4]In vitro: protective role of neferine against hypoxia induces oxidative stress by reactive oxygen species (ROS) scavenging and prevention of NF-κB nuclear translocation. Pretreatment with neferine inhibits autophagy induction by activating Akt/mTOR pathway and down regulating Beclin 1, PI3KCIII and LC3B-II in cells exposed to hypoxia. [1]Nef inhibits NF-κB there by inhibiting the expression of its downstream regulator COX-2. Nef significantly inhibited the ROS dependent mitochondrial mediated apoptosis induced during hypoxia. [2] Nferine inhibits ceardiac fibroblast proliferation, migration, and differentiation into myofibroblasts. neferine reduced high-glucose-induced collagen production and inhibited TGF-β1-Smad, ERK and p38 MAPK signaling activation in cardiac fibroblasts. [3]In vivo: Neferine prevented diabetes-induced cardiacfibrosis in vivo. [3] Neferine shows stronger blocking effect in rat neonatal CMs than liensinine. The IC50 of neferine was 1.25 uM on neonatal rat CMs. [4]

Reference

[1]. Baskaran R et al. Neferine prevents autophagy induced by hypoxia through activation of Akt/mTOR pathway and Nrf2 in muscle cells. Biomed Pharmacother. 2016 Aug 29;83:1407-1413.

[2]. Baskaran R et al. Neferine prevents NF-κB translocation and protects muscle cells from oxidative stress and apoptosis induced by hypoxia. Biofactors. 2016 Jul 8;42(4):407-17.

[3]. Liu X et al. Neferine inhibits proliferation and collagen synthesis induced by high glucose in cardiac fibroblasts and reduces cardiac fibrosis in diabetic mice. Oncotarget. 2016 Aug 11.

[4]. Yu Y et al. Liensinine- and Neferine-Induced Cardiotoxicity in Primary Neonatal Rat Cardiomyocytes and Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Int J Mol Sci. 2016 Jan 29;17(2).