Nitro Blue Tetrazolium (chloride)

• CAT Number: R065509
• CAS Number: 298-83-9
• Molecular Formula: C40H30N10O6 • 2Cl
• Molecular Weight: 817.6
• Purity: 98%
• Synonyms: NBT;p-Nitro Blue Tetrazolium;Nitrotetrazolium Blue;NSC 27622
• Storage: 2-8°C
• IUPAC Name: 2-[2-methoxy-4-[3-methoxy-4-[3-(4-nitrophenyl)-5-phenyltetrazol-2-ium-2-yl]phenyl]phenyl]-3-(4-nitrophenyl)-5-phenyltetrazol-2-ium;dichloride
• InChI: InChI=1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
• InChIKey: FSVCQIDHPKZJSO-UHFFFAOYSA-L
• SMILES: COC1=C(C=CC(=C1)C2=CC(=C(C=C2)[N+]3=NC(=NN3C4=CC=C(C=C4)[N+](=O)[O-])C5=CC=CC=C5)OC)[N+]6=NC(=NN6C7=CC=C(C=C7)[N+](=O)[O-])C8=CC=CC=C8.[Cl-].[Cl-]

Nitro Blue Tetrazolium (chloride)（Cat No.:R065509）, is a chemical compound commonly used in biochemical and immunohistochemical assays. It is a yellow to orange-red crystalline powder. Nitro Blue Tetrazolium (NBT) is often employed as a substrate for detecting enzyme activity, particularly in the study of redox reactions. In biological and immunological research, NBT is utilized to visualize the presence of enzymes like alkaline phosphatase and dehydrogenases. When these enzymes are active, they reduce NBT to a visible, insoluble formazan product, which produces a blue or purple coloration. This reaction aids in the identification and localization of specific enzymes and antigens in biological samples.

Reference

1.Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).