Nitrocefin

• CAT Number: R039865
• CAS Number: 41906-86-9
• Molecular Formula: C21H16N4O8S2
• Molecular Weight: 516.49
• Purity: ≥95%
• Synonyms: (6R,7R)-3-[(1E)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid; [6R-[3(E),6α,7β]]-3-[2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-
• Target: Beta-lactamase
• Storage: -20°C
• IUPAC Name: (6R,7R)-3-[(E)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
• InChI: InChI=1S/C21H16N4O8S2/c26-16(9-14-2-1-7-34-14)22-17-19(27)23-18(21(28)29)12(10-35-20(17)23)4-3-11-5-6-13(24(30)31)8-15(11)25(32)33/h1-8,17,20H,9-10H2,(H,22,26)(H,28,29)/b4-3+/t17-,20-/m1/s1
• InChIKey: LHNIIDJCEODSHA-OQRUQETBSA-N
• SMILES: C1C(=C(N2[C@H](S1)[C@@H](C2=O)NC(=O)CC3=CC=CS3)C(=O)O)/C=C/C4=C(C=C(C=C4)[N+](=O)[O-])[N+](=O)[O-]

Nitrocefin（Cat No.:R039865）is a high-purity chromogenic cephalosporin substrate widely used in microbiological and biochemical research. It is specifically designed to detect β-lactamase activity by undergoing a color change from yellow to red upon hydrolysis. This property makes Nitrocefin an essential tool for identifying β-lactamase-producing bacteria and assessing the efficacy of β-lactamase inhibitors. Its high sensitivity and specificity enable rapid screening in clinical diagnostics and antibiotic resistance studies. Nitrocefin is crucial for advancing research on bacterial resistance mechanisms and optimizing antimicrobial therapies.

Reference

O/’Callaghan, Cynthia H. et. al. /Novel Method for Detection of B-Lactamases by Using a Chromogenic Cephalosporin Substrate./ Antimicrobial Agents and Chemotherapy 1.4 (1972): 283-88. Ncbi.gov Web. 10 Oct. 2012.Parr, T. R., Jr. /Simple Screening Method for Beta-lactamase-positive and -negative Ampicillin-resistant Haemophilus Influenzae Isolates./ Journal of Clinical Microbiology 20.1 (1984): 131-32. Ncbi.gov. Web. 10 Oct. 2012.