For research use only. Not for therapeutic Use.
Nocodazole（Cat No.:I003117）is a small-molecule inhibitor that disrupts microtubule dynamics by binding to tubulin and preventing microtubule polymerization. This disruption halts mitosis, leading to cell cycle arrest in the G2/M phase. Nocodazole is widely used in cell biology research to study cell division, microtubule function, and mitotic spindle formation. It is also used to synchronize cells at specific cell cycle stages for various experimental purposes. Due to its ability to induce mitotic arrest, Nocodazole has potential applications in cancer research, where dysregulated cell division is a key factor.
| Catalog Number | I003117 |
| CAS Number | 31430-18-9 |
| Synonyms | methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate |
| Molecular Formula | C₁₄H₁₁N₃O₃S |
| Purity | ≥95% |
| Target | Autophagy |
| Solubility | DMSO 7 mg/ml; Water <1 mg/ml |
| Storage | 3 years -20C powder |
| IC50 | 0.21 uM (Abl) |
| IUPAC Name | methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate |
| InChI | InChI=1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19) |
| InChIKey | KYRVNWMVYQXFEU-UHFFFAOYSA-N |
| SMILES | COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3 |
| Reference | </br>1:Nocodazole treatment interrupted Brucella abortus invasion in RAW 264.7 cells, and successfully attenuated splenic proliferation with enhanced inflammatory response in mice. Reyes AW, Hop HT, Arayan LT, Huy TX, Min W, Lee HJ, Chang HH, Kim S.Microb Pathog. 2017 Feb;103:87-93. doi: 10.1016/j.micpath.2016.11.028. Epub 2016 Dec 23. PMID: 28017899 </br>2:An RNA-binding protein, RNP-1, protects microtubules from nocodazole and localizes to the leading edge during cytokinesis and cell migration in Dictyostelium cells. Ngo T, Miao X, Robinson DN, Zhou QQ.Acta Pharmacol Sin. 2016 Nov;37(11):1449-1457. doi: 10.1038/aps.2016.57. Epub 2016 Aug 29. PMID: 27569394 Free PMC Article</br>3:Nocodazole Induced Suicidal Death of Human Erythrocytes. Signoretto E, Honisch S, Briglia M, Faggio C, Castagna M, Lang F.Cell Physiol Biochem. 2016;38(1):379-92. doi: 10.1159/000438638. Epub 2016 Jan 29. PMID: 26824457 Free Article</br>4:Death-associated protein kinase 2 mediates nocodazole-induced apoptosis through interaction with tubulin. Isshiki K, Hirase T, Matsuda S, Miyamoto K, Tsuji A, Yuasa K.Biochem Biophys Res Commun. 2015 Dec 4-11;468(1-2):113-8. doi: 10.1016/j.bbrc.2015.10.151. Epub 2015 Oct 31. PMID: 26529546 </br>5:EDD, a ubiquitin-protein ligase of the N-end rule pathway, associates with spindle assembly checkpoint components and regulates the mitotic response to nocodazole. Scialpi F, Mellis D, Ditzel M.J Biol Chem. 2015 May 15;290(20):12585-94. doi: 10.1074/jbc.M114.625673. Epub 2015 Apr 1. PMID: 25833949 Free PMC Article</br>6:Novel hybrid nocodazole analogues as tubulin polymerization inhibitors and their antiproliferative activity. Kale SS, Jedhe GS, Meshram SN, Santra MK, Hamel E, Sanjayan GJ.Bioorg Med Chem Lett. 2015 May 1;25(9):1982-5. doi: 10.1016/j.bmcl.2015.03.019. Epub 2015 Mar 14. PMID: 25817588 Free PMC Article</br>7:[Microtubular disrupter nocodazole and vesicular transport inhibitor brefeldin A attenuate the glutoxim effect on Na+ transport in frog skin]. Mel/’nitskaia AV, Krutetskaia ZI, Butov SN, Kutetskaia NI, Antonov VG.Biofizika. 2014 Sep-Oct;59(5):883-6. Russian. PMID: 25730968 </br>8:Dominant lethal effects of nocodazole in germ cells of male mice. Attia SM, Ahmad SF, Okash RM, Bakheet SA.Food Chem Toxicol. 2015 Mar;77:101-4. doi: 10.1016/j.fct.2015.01.004. Epub 2015 Jan 13. PMID: 25595372 </br>9:Investigation of the effect of nocodazole on the PI3K/Akt signaling pathway in COS7 cells. Shi H, Wu W, Luo J.Mol Med Rep. 2014 Dec;10(6):3233-40. doi: 10.3892/mmr.2014.2632. Epub 2014 Oct 14. PMID: 25323455 </br>10:[Efficacy of nocodazole et experimental invasion Echinococcus granulosus of white mice]. Shkoliar NA, Kukhaleva IV, Legon/’kov IuA, Kovalenko FP.Med Parazitol (Mosk). 2014 Apr-Jun;(2):42-6. Russian. PMID: 25296427 |
