p-Nitrophenyl β-D-Glucopyranoside

• CAT Number: R002939
• CAS Number: 2492-87-7
• Molecular Formula: C₁₂H₁₅NO₈
• Molecular Weight: 301.25
• Purity: ≥95%
• Synonyms: 4-Nitrophenyl β-D-Glucopyranoside; PNPG
• Storage: Store at +4°C
• IUPAC Name: (2R,3S,4S,5R,6S)-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxane-3,4,5-triol
• InChI: InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11-,12-/m1/s1
• InChIKey: IFBHRQDFSNCLOZ-RMPHRYRLSA-N
• SMILES: C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O

p-Nitrophenyl β-D-glucopyranoside (Cat No.:R002939) is a chromogenic substrate commonly used in enzymatic assays to detect and quantify the activity of β-glucosidase enzymes. Upon hydrolysis by β-glucosidase, pNPG is converted into p-nitrophenol, a yellow compound that can be detected spectrophotometrically at 405 nm. This assay is widely used in the study of various biological processes, including plant cell wall degradation, microbial metabolism, and drug development. pNPG assays are sensitive, rapid, and relatively easy to perform, making them valuable tools in enzymology and related fields.