para-iodoHoechst 33258

• CAT Number: I002008
• CAS Number: 158013-43-5
• Molecular Formula: C25H23IN6
• Molecular Weight: 534.39
• Purity: ≥95%
• Synonyms: 2/’-(4-iodophenyl)-6-(4-methylpiperazin-1-yl)-1H,3/’H-2,5/’-bibenzo[d]imidazole
• Target: DNA Stain
• Solubility: DMSO: ≤ 20.5 mg/mL, Protect from light
• Storage: Store at -20°C

Para-iodoHoechst 33258 (CAT: I002008) is a member of the Hoechst family of blue fluorescent dyes commonly used for DNA staining. Specifically, Hoechst 33258 is a cell dye that is utilized for DNA quantitation. It binds to the minor groove of double-stranded DNA, resulting in a fluorescence emission that allows for the visualization and measurement of DNA content in cells. This dye is widely employed in various biological and biomedical research applications, including cell cycle analysis, DNA staining in microscopy, and flow cytometry. Its ability to selectively bind to DNA makes it a valuable tool for studying cellular processes and genetic material.

Reference

[1]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4/’,6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68.
[2]. a b c /Hoechst Stains/. Invitrogren (Molecular Probes).
[3]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4/’,6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68.