PF-543

• CAT Number: I001588
• CAS Number: 1415562-82-1
• Molecular Formula: C27H31NO4S
• Molecular Weight: 465.6
• Purity: ≥95%
• Synonyms: [(2R)-1-[[4-[[3-(benzenesulfonylmethyl)-5-methylphenoxy]methyl]phenyl]methyl]pyrrolidin-2-yl]methanol
• Target: S1P receptor
• Solubility: 10 mM in DMSO
• Storage: 3 years -20C powder
• IC50: 3.6 nM (Ki)
• InChIKey: NPUXORBZRBIOMQ-RUZDIDTESA-N

PF-543 is a novel cell-permeant inhibitor of SphK1 with a Ki of 3.6 nM, PF-543 is sphingosine-competitive and is more than 100-fold selective for SphK1 over the SphK2 isoform.IC50 value: 3.6 nM (Ki)Target: SphK1PF-543 is the most potent inhibitor of SphK1 described to date and it will be useful for dissecting specific roles of SphK1-driven S1P signalling.

Reference

[1]. Schnute ME, McReynolds MD, Kasten T et al. Modulation of cellular S1P levels with a novel, potent and specific inhibitor of sphingosine kinase-1. Biochem J. 2012 May 15;444(1):79-88.
[2]. Lynch KR. Building a better sphingosine kinase-1 inhibitor. Biochem J. 2012 May 15;444(1):e1-2.