For research use only. Not for therapeutic Use.
PFB-FDGlu is a selective lysosomal Glucocerebrosidase (GCase) substrate, which is metabolised by GCase to yield fluorescein. PFB-FDGlu is cell permeable and can be used with a flow cytometer to measure GCase activity in living cells on a single-cell basis[1].
PFB-FDGlu is cell permeable and can be used with a flow cytometer to measure GCase activity in living cells on a single-cell basis[1].
| Catalog Number | I044398 |
| CAS Number | 209540-62-5 |
| Synonyms | 2,3,4,5,6-pentafluoro-N-[3-oxo-3′,6′-bis[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]spiro[2-benzofuran-1,9′-xanthene]-5-yl]benzamide |
| Molecular Formula | C39H32F5NO16 |
| Purity | ≥95% |
| InChI | InChI=1S/C39H32F5NO16/c40-24-23(25(41)27(43)28(44)26(24)42)35(54)45-12-1-4-16-15(7-12)36(55)61-39(16)17-5-2-13(56-37-33(52)31(50)29(48)21(10-46)59-37)8-19(17)58-20-9-14(3-6-18(20)39)57-38-34(53)32(51)30(49)22(11-47)60-38/h1-9,21-22,29-34,37-38,46-53H,10-11H2,(H,45,54)/t21-,22-,29-,30-,31+,32+,33-,34-,37-,38-/m1/s1 |
| InChIKey | YYMJMZGTQFITLS-WXKYVOLFSA-N |
| SMILES | C1=CC2=C(C=C1NC(=O)C3=C(C(=C(C(=C3F)F)F)F)F)C(=O)OC24C5=C(C=C(C=C5)OC6C(C(C(C(O6)CO)O)O)O)OC7=C4C=CC(=C7)OC8C(C(C(C(O8)CO)O)O)O |
| Reference | [1]. Hughes LP, et al. Flow Cytometry Measurement of Glucocerebrosidase Activity in Human Monocytes. Bio Protoc. 2020;10(7):e3572. |
