For research use only. Not for therapeutic Use.
Pluripotin (Cat No.:I005030) is a dual inhibitor of ERK1 and RasGAP, with Kd values of 98nM and 212nM, respectively. Can be used to maintain the self-renewal of embryonic stem cells. Pluripotin also inhibits RSK1, RSK2, RSK3 and RSK4 with IC50 values of 0.5µM, 2.5µM, 3.3µM and 10.0µM, respectively.
| Catalog Number | I005030 |
| CAS Number | 839707-37-8 |
| Synonyms | (Z)-N-(3-(7-((1,3-dimethyl-1H-pyrazol-5-yl)amino)-1-methyl-2-oxo-1,2-dihydropyrimido[4,5-d]pyrimidin-3(4H)-yl)-4-methylphenyl)-3-(trifluoromethyl)benzimidic acid |
| Molecular Formula | C27H25F3N8O2 |
| Purity | ≥95% |
| Target | ERK |
| Solubility | DMSO: ＞ 34 mg/mL |
| Storage | -20°C |
| IC50 | 2.5±1.8 μM (EC50) |
| IUPAC Name | N-[3-[7-[(2,5-dimethylpyrazol-3-yl)amino]-1-methyl-2-oxo-4H-pyrimido[4,5-d]pyrimidin-3-yl]-4-methylphenyl]-3-(trifluoromethyl)benzamide |
| InChI | InChI=1S/C27H25F3N8O2/c1-15-8-9-20(32-24(39)17-6-5-7-19(11-17)27(28,29)30)12-21(15)38-14-18-13-31-25(34-23(18)36(3)26(38)40)33-22-10-16(2)35-37(22)4/h5-13H,14H2,1-4H3,(H,32,39)(H,31,33,34) |
| InChIKey | NBZFRTJWEIHFPF-UHFFFAOYSA-N |
| SMILES | CC1=C(C=C(C=C1)NC(=O)C2=CC(=CC=C2)C(F)(F)F)N3CC4=CN=C(N=C4N(C3=O)C)NC5=CC(=NN5C)C |
| Reference | </br>1:A small molecule (pluripotin) as a tool for studying cancer stem cell biology: proof of concept. Mertins SD, Scudiero DA, Hollingshead MG, Divelbiss RD Jr, Alley MC, Monks A, Covell DG, Hite KM, Salomon DS, Niederhuber JE.PLoS One. 2013;8(2):e57099. doi: 10.1371/journal.pone.0057099. Epub 2013 Feb 21. PMID: 23437320 Free PMC Article</br>2:Pluripotin enhances the expansion of rabbit limbal epithelial stem/progenitor cells in vitro. Duan H, Wang Y, Yang L, Qu M, Wang Q, Shi W, Zhou Q.Exp Eye Res. 2012 Jul;100:52-8. doi: 10.1016/j.exer.2012.04.012. Epub 2012 Apr 30. PMID: 22564971 </br>3:Efficient and user-friendly pluripotin-based derivation of mouse embryonic stem cells. Pieters T, Haenebalcke L, Hochepied T, D/’Hont J, Haigh JJ, van Roy F, van Hengel J.Stem Cell Rev. 2012 Sep;8(3):768-78. doi: 10.1007/s12015-011-9323-x. PMID: 22011883 Free PMC Article</br>4:The use of SC1 (Pluripotin) to support mESC self-renewal in the absence of LIF. Xiong W, Gao Y, Cheng X, Martin C, Wu D, Yao S, Kim MJ, Liu Y.J Vis Exp. 2009 Nov 18;(33). pii: 1550. doi: 10.3791/1550. PMID: 19924098 Free PMC Article</br>5:Pluripotin combined with leukemia inhibitory factor greatly promotes the derivation of embryonic stem cell lines from refractory strains. Yang W, Wei W, Shi C, Zhu J, Ying W, Shen Y, Ye X, Fang L, Duo S, Che J, Shen H, Ding S, Deng H.Stem Cells. 2009 Feb;27(2):383-9. doi: 10.1634/stemcells.2008-0974. PMID: 19056907 Free Article |
