PNPP

• CAT Number: I020703
• CAS Number: 4264-83-9
• Molecular Formula: C₆H₄NNa₂O₆P
• Molecular Weight: 263.05
• Purity: ≥95%
• IUPAC Name: disodium;(4-nitrophenyl) phosphate
• InChI: InChI=1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
• InChIKey: VIYFPAMJCJLZKD-UHFFFAOYSA-L
• SMILES: C1=CC(=CC=C1[N+](=O)[O-])OP(=O)([O-])[O-].[Na+].[Na+]

PNPP(CAT: I020703) (p-Nitrophenyl Phosphate) is a widely used non-proteinaceous chromogenic substrate for detecting the activity of alkaline and acid phosphatases in ELISA and spectrophotometric assays. Phosphatases catalyze the hydrolysis of PNPP, releasing inorganic phosphate and para-nitrophenol (pNP). Under alkaline conditions, pNP is converted to its conjugate base, producing a yellow color that can be quantitatively measured at 405 nm. This colorimetric change allows for sensitive and accurate detection of phosphatase activity, making PNPP an essential tool in biochemical assays, enzyme kinetics studies, and diagnostic applications.