Poly-D-lysine hydrobromide (MW 30000-70000)

• CAT Number: I044436
• CAS Number: 27964-99-4
• Molecular Formula: (C6H14N2O2)x.xHBr
• Molecular Weight: 30000-70000
• Purity: ≥95%

Poly-D-lysine hydrobromide (MW 30000-70000) is a synthetic polymeric substrate and is one of the most widely used substrate in neural cell culture. Poly-D-lysine hydrobromide (MW 30000-70000) removes proteinase K-resistant PrP from prion-infected SN56 neuroblastoma cells without affecting PrPC[1][2].
Poly-D-lysine is immunogenic in rabbits only when injected at low doses[1].
The approximate concentration of Poly-D-lysine (PDL) needed to lower the amount of PrPres by 50% corresponds to ~0.8 μg/mL or 50 nM[2].

Reference

[1]. HansVermeersch, et al. Immunogenicity of poly-D-lysine, a potential polymeric drug carrier. Journal of Controlled Release. Volume 32, Issue 3, 1 December 1994, Pages 225-229.

[2]. Zhou Xu, et al. Mechanistic insights into cellular alteration of prion by poly-D-lysine: the role of H2H3 domain. FASEB J. 2011 Oct;25(10):3426-35.
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