Ponceau S Staining Solution

• CAT Number: I011140
• CAS Number: 6226-79-5
• Molecular Formula: C22H12N4O13S4 • 4Na
• Molecular Weight: 760.6
• Purity: ≥95%
• Synonyms: 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt
• Solubility: Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution)
• Storage: Store at RT
• IUPAC Name: tetrasodium;3-hydroxy-4-[[2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl]diazenyl]naphthalene-2,7-disulfonate
• InChI: InChI=1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4
• InChIKey: KQHKSGRIBYJYFX-UHFFFAOYSA-J
• SMILES: C1=CC(=CC=C1N=NC2=CC(=C(C=C2)N=NC3=C4C=CC(=CC4=CC(=C3O)S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+]

Ponceau S（Cat No.:I011140）, also known as Acid Red 112, is a widely used staining agent in Western blotting techniques. It is typically utilized in an acidic aqueous solution and is compatible with antibody-antigen binding. When applied to protein-loaded membranes, Ponceau S selectively stains proteins, causing them to appear red. This staining step serves as a visual indicator of protein transfer efficiency and can be used to confirm successful protein transfer onto the membrane before proceeding with antibody probing or other detection methods in Western blot analysis.