For research use only. Not for therapeutic Use.
Puromycin Dihydrochloride（Cat No.:R059263）is an aminonucleoside antibiotic derived from Streptomyces alboniger, widely used in research for its ability to inhibit protein synthesis. It mimics aminoacyl-tRNA, incorporating itself into growing polypeptide chains and causing premature chain termination, which effectively halts translation. Due to this mechanism, Puromycin is valuable in molecular biology studies, particularly for selecting cells with resistance markers in genetic experiments. Additionally, it has applications in studying cellular processes like apoptosis, autophagy, and translation dynamics, providing insights into protein synthesis and cell regulation in diverse biological systems.
| Catalog Number | R059263 |
| CAS Number | 58-58-2 |
| Synonyms | (S)-2-amino-N-((2S,3R,4S,5R)-5-(6-(dimethylamino)-9H-purin-9-yl)-4-hydroxy-2-(hydroxymethyl)tetrahydrofuran-3-yl)-3-(4-methoxyphenyl)propanamide dihydrochloride |
| Molecular Formula | C₂₂H₂₉N₇O₅ · 2HCl |
| Purity | ≥95% |
| Target | Bacterial |
| Solubility | >27.2mg/mL in DMSO |
| Storage | Store at -20C |
| IUPAC Name | (2S)-2-amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide;dihydrochloride |
| InChI | InChI=1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1 |
| InChIKey | MKSVFGKWZLUTTO-FZFAUISWSA-N |
| SMILES | CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl |
| Reference | <p>Azzam, M. E. /Mechanism of Puromycin Action: Fate of Ribosomes after Release of Nascent Protein Chains from Polysomes./ <em>PNAS</em> 70.12 (1973): 3866-3869. <em>www.ncbi.gov</em>. Web. 4 Sept. 2012.</p><p>Vara, J. /Cloning and Expression of a Puromycin N-acetyl Transferase Gene from Streptomyces Alboniger in Streptomyces Lividans and Escherichia Coli./ Gene 33.2 (1985): 195-206. Www.ncbi.gov. Web. 7 Sept. 2012.</p><p>Algire, Mikkel A. /New Selectable Marker for Manipulating the Simple Genomes of Mycoplasma Species./ Antimicrobial Agents and Chemotherapy 53.10 (2009): 4429-432. Ncbi.nlm.nih.gov. Web. 5 Nov. 2014.</p></span></p> |
