Pyronine B

For research use only. Not for therapeutic Use.

  • CAT Number: I035273
  • CAS Number: 2150-48-3
  • Molecular Formula: C21H27ClN2O
  • Molecular Weight: 358.91
  • Purity: 98%
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Pyronine B(Cat No.:I035273), is a synthetic xanthene dye widely used as a staining agent in histology and cytology. It is characterized by its vibrant red color and has a specific affinity for nucleic acids, especially RNA, within cells. Pyronine B finds application in various fields, including fluorescence microscopy and as a counterstain in combination with other dyes. Its ability to selectively stain nucleic acids makes it a valuable tool for visualizing and studying cellular structures, aiding in biological research, and diagnostic applications.


Catalog Number I035273
CAS Number 2150-48-3
Synonyms

Pyronine B; NSC 44690; NSC-44690; NSC44690.

Molecular Formula C21H27ClN2O
Purity 98%
Target DNA Stain
Solubility Soluble in DMSO
Appearance Solid powder
Storage Store at RT
IUPAC Name 3,6-Bis(diethylamino)xanthylium chloride
InChI InChI=1S/C21H27N2O.ClH/c1-5-22(6-2)18-11-9-16-13-17-10-12-19(23(7-3)8-4)15-21(17)24-20(16)14-18;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1
InChIKey CXZRDVVUVDYSCQ-UHFFFAOYSA-M
SMILES CCN(C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)C=C2C=C1)CC.[Cl-]
Reference

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4: Chen WX, Zhu HL, Xue M, Zhou H, Zhao F, Yan N, Chen Y. Quick staining technique for myeloperoxidase using potassium iodide and oxidized pyronine B. Acta Histochem. 2014 Jan;116(1):292-6. doi: 10.1016/j.acthis.2013.08.001. Epub 2013 Sep 14. PubMed PMID: 24044784.
5: Ulusoy Hİ, Yılmaz O, Gürkan R. A micellar improved method for trace levels selenium quantification in food samples, alcoholic and nonalcoholic beverages through CPE/FAAS. Food Chem. 2013 Aug 15;139(1-4):1008-14. doi: 10.1016/j.foodchem.2013.01.087. Epub 2013 Feb 8. PubMed PMID: 23561203.
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11: Bordello J, Reija B, Al-Soufi W, Novo M. Host-assisted guest self-assembly: enhancement of the dimerization of pyronines Y and B by gamma-cyclodextrin. Chemphyschem. 2009 Apr 14;10(6):931-9. doi: 10.1002/cphc.200800776. PubMed PMID: 19294686.
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13: Villagra NA, Hidalgo AA, Santiviago CA, Saavedra CP, Mora GC. SmvA, and not AcrB, is the major efflux pump for acriflavine and related compounds in Salmonella enterica serovar Typhimurium. J Antimicrob Chemother. 2008 Dec;62(6):1273-6. doi: 10.1093/jac/dkn407. Epub 2008 Sep 26. PubMed PMID: 18819967.
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15: Rezaei B, Majidi N. A simple and high sensitive spectrophotometric method for ultra trace determination of ruthenium with its catalytic effect on the oxidation of pyronin B by periodate. Spectrochim Acta A Mol Biomol Spectrosc. 2007 Apr;66(4-5):869-73. Epub 2006 May 10. PubMed PMID: 16872868.
16: Reija B, Al-Soufi W, Novo M, Vazquez Tato J. Specific interactions in the inclusion complexes of pyronines Y and B with beta-cyclodextrin. J Phys Chem B. 2005 Feb 3;109(4):1364-70. PubMed PMID: 16851104.
17: Gao F, Li YX, Zhang L, Wang L. Cetyltrimethylammonium bromide sensitized resonance light-scattering of nucleic acid–Pyronine B and its analytical application. Spectrochim Acta A Mol Biomol Spectrosc. 2004 Sep;60(11):2505-9. PubMed PMID: 15294236.
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