R-1479

• CAT Number: I003673
• CAS Number: 478182-28-4
• Molecular Formula: C9H12N6O5
• Molecular Weight: 284.23
• Purity: ≥95%
• Synonyms: 4-amino-1-[(2R,3R,4S,5R)-5-azido-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidin-2-one
• Target: HCV
• Solubility: 10 mM in DMSO
• Storage: Store at -20°C
• IC50: 1.28 uM (HCV replicon assay) [1]

R1479 (4/’-azidocytidine), is a potent and highly specific inhibitor of HCV replication in cell culture, and that its triphosphate is a potent and highly selective inhibitor of NS5B mediated RNA synthesis, the HCV encoded RNA polymerase.IC50 Value: 1.28 uM (HCV replicon assay) [1]Target: HCVin vitro: R1479 (4/’-azidocytidine) is a specific inhibitor of HCV replication in the HCV subgenomic replicon system (IC(50) = 1.28 microM) with similar potency compared with 2/’-C-methylcytidine (IC(50) = 1.13 microM). R1479 showed no effect on cell viability or proliferation of HCV replicon or Huh-7 cells at concentrations up to 2 mM. HCV replicon RNA could be fully cleared from replicon cells after prolonged incubation with R1479 [2].in vivo:Toxicity: R1479 displayed good activity in the replicon assay with no measurable cytotoxic or cytostatic effect [1].Clinical trial: A Study of Balapiravir in Patients With Dengue Virus Infection. Phase 1

Reference

[1]. Smith DB, et al. Design, synthesis, and antiviral properties of 4/’-substituted ribonucleosides as inhibitors of hepatitis C virus replication: the discovery of R1479. Bioorg Med Chem Lett. 2007 May 1;17(9):2570-6.
[2]. Klumpp K, et al. The novel nucleoside analog R1479 (4/’-azidocytidine) is a potent inhibitor of NS5B-dependent RNA synthesis and hepatitis C virus replication in cell culture. J Biol Chem. 2006 Feb 17;281(7):3793-9.