Recombinant Kex2 protease

• CAT Number: I040143
• CAS Number: 99676-46-7
• Purity: ≥95%

Recombinant Kex2 protease is a membrane-bound, Ca2+-dependent serine protease. Recombinant Kex2 protease specifically recognize and cleave the carboxyl-terminal peptide bonds of dibasic amino acids[1].
Protocol
1) Reaction buffer: 50 mM Tris-HCl, 2 mM CaCl2 (pH 7.0-9.0).
2) Storage buffer: 20 mM (pH 5.2) NaAc-HAc, 2 mM CaCl2. If not used immediately after dissolution, the product can be dissolved in storage buffer. The final concentration of the enzyme after dissolution is about 1-10mg/ml, according to It needs to be aliquoted and stored below -20℃.
3) Reaction conditions: The optimal reaction pH is 9.0, and the stable pH is 5.0-6.0.

Reference

[1]. Redding K, et, al. Immunolocalization of Kex2 protease identifies a putative late Golgi compartment in the yeast Saccharomyces cerevisiae. J Cell Biol. 1991 May;113(3):527-38.
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