Resorufin butyrate

• CAT Number: M082028
• CAS Number: 15585-42-9
• Molecular Formula: C16H13NO4
• Molecular Weight: 283.28
• Purity: ≥95%
• Synonyms: (7-oxophenoxazin-3-yl) butanoate
• InChI: InChI=1S/C16H13NO4/c1-2-3-16(19)20-11-5-7-13-15(9-11)21-14-8-10(18)4-6-12(14)17-13/h4-9H,2-3H2,1H3
• InChIKey: RGJTZDRUTUWWTD-UHFFFAOYSA-N
• SMILES: CCCC(=O)OC1=CC2=C(C=C1)N=C3C=CC(=O)C=C3O2

Resorufin butyrate is a fluorogenic substrate for triglyceride lipases and cholinesterase (Ex=570 nm, Em=580 nm)[1][2][3].
Resorufin butyrate (2.3 μM) displays a linear increase in fluorescence over 60 min (conducted in 384-well plates using 12 nM of bLPL enzyme and substrate in PBS, pH 7.5, 5% DMSO)[1].

Reference

[1]. J Biomol Screen, et al. Resorufin butyrate as a soluble and monomeric high-throughput substrate for a triglyceride lipase. J Biomol Screen. 2012 Feb;17(2):245-51.
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[2]. Guilbault, G.G., et al. Resorufin butyrate and indoxyl acetate as fluorogenic substrates for chloinesterase. Anal Chem. 1965 Jan;37:120-3.
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[3]. J.Hofmann, et al. Immobilized enzyme kinetics analyzed by flow-through microfluorimetry : Resorufin-β-d-galactopyranoside as a New Fluorogenic Substrate for β-Galactosidase. Anal. Chim. Acta 163, 67-72 (1984).