For research use only. Not for therapeutic Use.
Ribonuclease A (EC 3.1.27.5) cleaves RNA 3′ to pyrimidines and actively cleaves RNA at every pyrimidine residue. Ribonuclease A catalyzes the hydrolysis of single stranded RNA in the absence of metal ions or cofactors[1][2][3].
A major application of RNase A is the removal of RNA from plasmid DNA preparations.
Preparation of RNase A stock solution
1. Use 10 mM sodium acetate (pH 5.2) to prepare 10 mg/mL RNase A stock solution;
2. Heat at 100 ℃ for 15 min;
3. Cool to room temperature, add 1/10（v/v） of 1 M Tris-HCl（pH 7.4）, adjust the pH to 7.4;
4. The solution can be stored at 20 ℃ for 2 years.
Note: When the RNase A solution is boiled under neutral conditions, RNase precipitate will form; if it is boiled at a lower pH, any precipitation may be caused by the presence of protein impurities. At this time, the precipitate can be removed by high-speed centrifugation (13 000 rpm).
| Catalog Number | I042665 |
| CAS Number | 9001-99-4 |
| Purity | ≥95% |
| Reference | [1]. Thakur P, et al. Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis. Methods Enzymol. 2021;658:1-24. [2]. Brown D, Pasloske BL. Ribonuclease-resistant RNA controls and standards. Methods Enzymol. 2001;341:648-54. [3]. Wallace JA, Shen JK. Predicting pKa values with continuous constant pH molecular dynamics. Methods Enzymol. 2009;466:455-75. |
